Novel heme ligand displacement by CO in the soluble hemophore HasA and its proximal ligand mutants: implications for heme uptake and release.

Biochemistry
Gudrun S Lukat-RodgersAnne Lecroisey

Abstract

HasASM, a hemophore secreted by the Gram-negative bacteria Serratia marcescens, extracts heme from host hemoproteins and shuttles it to HasRSM, a specific hemophore outer membrane receptor. Heme iron in HasASM is in a six-coordinate ferric state. It is linked to the protein by the heretofore uncommon axial ligand set, His32 and Tyr75. A third residue of the heme pocket, His83, plays a crucial role in heme ligation through hydrogen bonding to Tyr75. The vibrational frequencies of coordinated carbon monoxide constitute a sensitive probe of trans ligand field, FeCO structure, and electrostatic landscape of the distal heme pockets of heme proteins. In this study, carbonyl complexes of wild-type (WT) HasASM and its heme pocket mutants His32Ala, Tyr75Ala, and His83Ala were characterized by resonance Raman spectroscopy. The CO complexes of WT HasASM, HasASM(His32Ala), and HasASM(His83Ala) exhibit similar spectral features and fall above the line that correlates nuFe-CO and nuC-O for proteins having a proximal imidazole ligand. This suggests that the proximal ligand field in these CO adducts is weaker than that for heme-CO proteins bearing a histidine axial ligand. In contrast, the CO complex of HasASM(Tyr75Ala) has resonance Raman sig...Continue Reading

References

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Citations

Nov 10, 2011·Biochemistry·Brian San FranciscoRobert G Kranz
Sep 11, 2012·Biochemistry·Luciana HannibalDennis J Stuehr
Jun 16, 2012·The Journal of Biological Chemistry·Célia Caillet-SaguyAnne Lecroisey
Sep 7, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yu CaoMassimo Coletta
Jan 25, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Erin Sarah Honsa, Anthony William Maresso
May 21, 2013·FEBS Letters·Marco Antonio González-LópezJosé de Jesús Olivares-Trejo
May 27, 2014·Journal of Inorganic Biochemistry·Shin-Ichi OzakiKoichiro Ishimori
Jun 21, 2014·Journal of the American Chemical Society·Douglas P LinderKenton R Rodgers
Feb 13, 2015·Biochemistry·Daniel KalukaSyun-Ru Yeh
Oct 20, 2015·Biochemistry·Elizabeth B DraganovaDabney W Dixon
Feb 23, 2011·Current Opinion in Chemical Biology·Jeffery A MayfieldJennifer L DuBois
Aug 27, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Elizabeth B DraganovaDabney W Dixon
Apr 25, 2017·Biochemistry·Takeshi UchidaKoichiro Ishimori
May 14, 2020·International Journal of Molecular Sciences·Poorva GhoshLi Zhang
Aug 7, 2008·Journal of the American Chemical Society·Francesco P NicolettiGiulietta Smulevich

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