PMID: 2108022Feb 22, 1990Paper

Novel hyperglycosylated weak gelatin-binding fibronectin from human fetal placenta. Fractionation of a high poly(N-acetyllactosamine) fragment by tomato lectin affinity chromatography

European Journal of Biochemistry
B C Zhu, R A Laine

Abstract

A novel hyperglycosylated fraction of human term fetal placental fibronectin was detected by long-term affinity binding to gelatin-Sepharose. An 18-h batch-wise gelatin-binding step was necessary to obtain a very low-affinity binding fraction, characterized by especially high N-acetylglucosamine and galactose content, and diffuse, poorly stained Coomassie bands on SDS/polyacrylamide electrophoretograms. The presence of a high proportion of long 7-10-kDa poly(N-acetyllactosamine)-containing N-linked carbohydrate chains was confirmed by their gel permeation behavior, susceptibility to endo-beta-galactosidase and by methylation analysis. Our previous results suggest that 4.5-7-kDa poly(N-acetyllactosamine) structures reduce the binding of fibronectin and its chymotryptic Ala260-Trp599 subdomain GB44 to gelatin [Zhu, B. C. R. & Laine, R. A. (1985) J. Biol. Chem. 260, 4041-4045]. Based on a gradient of urea used to dissociate gelatin-bound GB44, in the present study, fractions containing the novel 7-10-kDa carbohydrates showed significantly weaker binding to gelatin. Weak gelatin-binding characteristics of this novel hyperglycosylated fraction suggest that extended poly(N-acetyllactosamine) N-linked chains can significantly weaken h...Continue Reading

References

Dec 13, 1977·Biochemistry·K M YamadaI Pastan
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Mar 15, 1985·The Biochemical Journal·B A Bray
Jan 1, 1987·Methods in Enzymology·R A LaineB C Zhu
Apr 29, 1983·Biochemical and Biophysical Research Communications·J FinneC Goridis
Jan 1, 1982·Methods in Enzymology·M S Nachbar, J D Oppenheim

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Citations

Jan 10, 1994·FEBS Letters·A LinnalaI Virtanen
Sep 17, 2009·Molecular BioSystems·Richard D Cummings
Sep 23, 2020·Advanced Drug Delivery Reviews·Jennifer Patten, Karin Wang

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