Novel immunosuppressive proteins purified from the serum of liver-retransplanted rats

Transplantation
S GotoN Kamada

Abstract

Liver grafts between certain rat strain combinations, such as DA (RT1a)-into-PVG (RT1c), are accepted without the use of immunosuppressive agents. To explore the nature and role of serum proteins in liver-induced immunosuppression, we have developed a retransplantation model of rat liver grafting. In this procedure, orthotopic liver transplantation (OLT) is carried out in the DA-into-PVG combination; two days later the DA liver is removed and a new PVG liver implanted into the same recipient (re-OLT). Serum from re-OLT rats was immunosuppressive when tested in mixed lymphocyte reactions (MLR). Three novel proteins were detected in re-OLT serum by SDS-PAGE, with sizes of 180 kD, 87 kD, and 10 kD. The N-terminal sequences of these were distinct and did not match protein sequences in the computer databases, although there was some homology between the 10 kD sequence and the beta-chain of rat hemoglobin. Purified 87 kD and 10 kD proteins were immunosuppressive in MLR; in both cases suppression was dose-dependent and nonstimulator-specific. Production of the 180 kD and 87 kD molecules required the presence of the recipient spleen. We conclude that re-OLT serum contains novel immunosuppressive proteins, which may be products of immun...Continue Reading

References

Mar 1, 1990·Journal of Biochemistry·T ShinomiyaN Kamada
Jul 1, 1990·Electrophoresis·T Choli, B Wittmann-Liebold
Aug 27, 1981·Nature·N KamadaB Roser
Jul 1, 1995·European Journal of Immunology·R M SutherlandY Paterson
Jan 1, 1995·Microsurgery·S GotoF Vari
Jan 1, 1995·Transplant International : Official Journal of the European Society for Organ Transplantation·J M DoutrelepontL De Pauw
Feb 1, 1985·Immunology Today·R A Robins, R W Baldwin

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Citations

Feb 26, 2005·Plastic and Reconstructive Surgery·Subhro K SenSusan E Mackinnon
May 22, 2013·Clinical & Developmental Immunology·Toshiaki NakanoShigeru Goto
Sep 27, 2000·Transplant Immunology·K C ChiangT Hashimoto
Mar 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kuei-Chen ChiangShuji Sato

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