Novel in situ visualisation of rat intestinal absorption of polyphenols via matrix-assisted laser desorption/ionisation mass spectrometry imaging

Scientific Reports
Huu-Nghi NguyenToshiro Matsui

Abstract

Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) is presently used in physiological evaluations for visualisation of targets in organs. In the present study, MALDI-MSI was used as a visualisation technique to investigate the intestinal absorption of polyphenols. Nifedipine/phytic acid-aided MALDI-MSI was performed to visualise theaflavin-3'-O-gallate (TF3'G) and epicatechin-3-O-gallate (ECG) in the rat jejunum for 50-µM, 60-min transport experiments. Non-absorbable TF3'G was successfully visualised at the apical region, whereas absorbable ECG was detected throughout the rat jejunum. MALDI-MSI was also performed to determine the transport routes of the target metabolites. Signals corresponding to TF3'G and ECG in the membranes were diminished following treatment with inhibitors targeting the monocarboxylic acid transporter and organic anion transporting polypeptides. Enhanced visualisation of TF3'G was achieved by inhibiting efflux routes. Our findings demonstrated that the present MALDI-MSI can provide critical spatial informations on intestinal absorption of targets, by which TF3'G and ECG were incorporated into intestinal tissues, followed by efflux back to the apical compartment. In addition,...Continue Reading

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Citations

May 8, 2020·Foods·Yoshimura Yukihiro, Nobuhiro Zaima
Aug 5, 2021·Journal of Agricultural and Food Chemistry·Yuki TakashimaShoko Kobayashi
Aug 9, 2019·Journal of Agricultural and Food Chemistry·Alexia M NectouxToshiro Matsui
Jul 11, 2020·Journal of Agricultural and Food Chemistry·Ye ZhangToshiro Matsui
Oct 26, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shoko MatsushitaYusuke Suzuki

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Software Mentioned

Bruker FlexAnalysis
ImagePrep
Bruker Data Analysis
Bruker FlexImaging

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