Novel in vivo-degradable cellulose-chitin copolymer from metabolically engineered Gluconacetobacter xylinus.

Applied and Environmental Microbiology
Vikas YadavDavid L Kaplan

Abstract

Despite excellent biocompatibility and mechanical properties, the poor in vitro and in vivo degradability of cellulose has limited its biomedical and biomass conversion applications. To address this issue, we report a metabolic engineering-based approach to the rational redesign of cellular metabolites to introduce N-acetylglucosamine (GlcNAc) residues into cellulosic biopolymers during de novo synthesis from Gluconacetobacter xylinus. The cellulose produced from these engineered cells (modified bacterial cellulose [MBC]) was evaluated and compared with cellulose produced from normal cells (bacterial cellulose [BC]). High GlcNAc content and lower crystallinity in MBC compared to BC make this a multifunctional bioengineered polymer susceptible to lysozyme, an enzyme widespread in the human body, and to rapid hydrolysis by cellulase, an enzyme commonly used in biomass conversion. Degradability in vivo was demonstrated in subcutaneous implants in mice, where modified cellulose was completely degraded within 20 days. We provide a new route toward the production of a family of tailorable modified cellulosic biopolymers that overcome the longstanding limitation associated with the poor degradability of cellulose for a wide range of p...Continue Reading

References

Jul 1, 1975·Macromolecules·F J Kolpak, J Blackwell
Dec 1, 1992·International Journal of Biological Macromolecules·R OgawaT Koriyama
Mar 1, 1991·Microbiological Reviews·P RossM Benziman
Jan 1, 1991·Critical Reviews in Microbiology·R E Cannon, S M Anderson
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H C WongA W Emerick
Aug 1, 1969·Science·D M Chipman, N Sharon
Dec 1, 1994·International Journal of Biological Macromolecules·A ShiraiS Tokura
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·T NakaiT Hayashi
Oct 16, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·Y Suzuki, Y Makino
Jun 5, 2003·Nature Biotechnology·Vincent J J MartinJay D Keasling
Sep 19, 2003·Applied Microbiology and Biotechnology·B S DienT W Jeffries
Aug 16, 2005·Biomaterials·Wojciech CzajaR Malcolm Brown
Nov 10, 2005·Journal of Biomedical Materials Research. Part a·Gisela HeleniusBo Risberg
Jul 15, 2006·Nature Biotechnology·Charlotte Schubert
Jan 9, 2007·Biomacromolecules·Wojciech K CzajaR Malcolm Brown
Aug 16, 2008·Nature·Edward M Rubin
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·A Joe ShawLee R Lynd
Oct 30, 2008·Journal of Molecular Microbiology and Biotechnology·Frank Reinecke, Alexander Steinbüchel
Nov 20, 2008·FEMS Microbiology Reviews·Konstantinos Michalodimitrakis, Mark Isalan

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Citations

Jan 20, 2016·Applied Microbiology and Biotechnology·Gazi Sakir HossainLong Liu
Nov 6, 2013·Carbohydrate Polymers·Guokui QinZhongyuan Sun David L Kaplan
Jun 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Michael FloreaTom Ellis
Jan 18, 2018·The Journal of Biological Chemistry·Lisa A AndersonKristala L J Prather
Jan 30, 2013·Macromolecular Bioscience·Sílvia GomesDavid L Kaplan
Jan 18, 2015·Applied and Environmental Microbiology·Yuri IshiiShinsuke Fujiwara
Jan 15, 2013·Journal of Tissue Engineering and Regenerative Medicine·Vikas YadavDavid L Kaplan
Apr 23, 2020·International Journal of Molecular Sciences·Nele Pascale GrigullSusanne Mayer-Wagner
Mar 7, 2019·Microbial Biotechnology·Raquel PortelaRita G Sobral
Mar 19, 2019·Microbial Biotechnology·Paulina JacekStanisław Bielecki
Dec 6, 2020·International Journal of Molecular Sciences·Amritpal SinghTom Ellis
Nov 26, 2020·Trends in Biotechnology·Wil V Srubar
Apr 16, 2021·Applied Microbiology and Biotechnology·Philipp Moritz FrickeTino Polen
May 7, 2021·International Journal of Biological Macromolecules·Mehran MoradiMehrdad Forough
Jul 3, 2021·Nanomaterials·Francisco G BlancoM Auxiliadora Prieto
Mar 14, 2019·ACS Synthetic Biology·Min Yan TehMeng How Tan
Sep 15, 2021·Bioengineered·Reeta Rani SinghaniaCheng Di Dong
Nov 19, 2021·Biotechnology & Genetic Engineering Reviews·Elahe Vadaye KheiryMohammad Amin Kerachian
Nov 26, 2021·Bioengineered·Reshmy RAshok Pandey

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