Novel insights into mannitol metabolism in the fungal plant pathogen Botrytis cinerea

The Biochemical Journal
Thierry DulermoPascale Cotton

Abstract

In order to redefine the mannitol pathway in the necrotrophic plant pathogen Botrytis cinerea, we used a targeted deletion strategy of genes encoding two proteins of mannitol metabolism, BcMTDH (B. cinerea mannitol dehydrogenase) and BcMPD (B. cinerea mannitol-1-phosphate dehydrogenase). Mobilization of mannitol and quantification of Bcmpd and Bcmtdh gene transcripts during development and osmotic stress confirmed a role for mannitol as a temporary and disposable carbon storage compound. In order to study metabolic fluxes, we followed conversion of labelled hexoses in wild-type and DeltaBcmpd and DeltaBcmtdh mutant strains by in vivo NMR spectroscopy. Our results revealed that glucose and fructose were metabolized via the BcMPD and BcMTDH pathways respectively. The existence of a novel mannitol phosphorylation pathway was also suggested by the NMR investigations. This last finding definitively challenged the existence of the originally postulated mannitol cycle in favour of two simultaneously expressed pathways. Finally, physiological and biochemical studies conducted on double deletion mutants (DeltaBcmpdDeltaBcmtdh) showed that mannitol was still produced despite a complete alteration of both mannitol biosynthesis pathways. T...Continue Reading

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Citations

Aug 5, 2014·Annual Review of Phytopathology·Darren Soanes, Thomas A Richards
Jul 3, 2013·Journal of Proteomics·Raquel González-FernándezJesús V Jorrín-Novo
Feb 7, 2016·Trends in Plant Science·Takshay K Patel, John D Williamson
Dec 19, 2012·Trends in Biotechnology·Na WeiYong-Su Jin
Sep 30, 2015·Fungal Genetics and Biology : FG & B·Scott GriffithsJérôme Collemare
Aug 22, 2014·Archives of Microbiology·Victoria E González-RodríguezFrancisco Javier Fernández-Acero
Sep 27, 2016·Phytochemistry·M Soledade C Pedras, Myung Ryeol Park
Jun 27, 2017·Fungal Genetics and Biology : FG & B·Julia Schumacher
Sep 28, 2014·Applied and Environmental Microbiology·Sana ThabetPascale Cotton
Oct 15, 2014·Journal of Proteome Research·Huizhuan YanSixue Chen

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