Novel interaction of selenium-binding protein with glyceraldehyde-3-phosphate dehydrogenase and fructose-bisphosphate aldolase of Arabidopsis thaliana

Functional Plant Biology : FPB
Adamantia AgalouAndreas Roussis

Abstract

The metabolic role and regulation of selenium, particularly in plants, is poorly understood. One of the proteins probably involved in the metabolic regulation of this element is the selenium-binding protein (SBP) with homologues present across prokaryotic and eukaryotic species. The high degree of conservation of SBP in different organisms suggests that this protein may play a role in fundamental biological processes. In order to gain insight into the biochemical function of SBP in plants we used the yeast two-hybrid system to identify proteins that potentially interact with an Arabidopsis thaliana (L.) Heynh. homologue. Among the putative binding partners of SBP, a NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and a fructose-bisphosphate aldolase (FBA) were found as reliable positive candidates. The interaction of these proteins with SBP was confirmed by in vitro binding assays. Previous findings in Escherichia coli, demonstrated the direct binding of selenium to both GAPDH and aldolase. Therefore our results reveal the interaction, at least in pairs, of three proteins that are possibly linked to selenium and suggest the existence of a protein network consisting of at least SBP, GAPDH and FBA, triggered by or...Continue Reading

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Citations

Jun 1, 2007·Functional Plant Biology : FPB·Rui A Gomes-JuniorRicardo A Azevedo
Jan 14, 2020·Plant Science : an International Journal of Experimental Plant Biology·Irene DervisiAndreas Roussis

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Datasets Mentioned

BETA
O23264
BAC53217
CAE71767
AAX39709
AK173579

Methods Mentioned

BETA
environmental stress
two hybrid
PCR
two-hybrid
pull down
pull-down

Software Mentioned

Blast
ClustalW
SWISSPROT
TrEMBL
BlastP

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