Novel ion mobility setup combined with collision cell and time-of-flight mass spectrometer

Journal of the American Society for Mass Spectrometry
Alexander V Loboda

Abstract

An ion mobility cell of a novel type was coupled to an orthogonal injection time-of-flight (TOF) mass spectrometer. The mobility cell operates at low-pressure and contains a segmented RF ion guide providing an axial electric field that drives the ions towards the exit. A flow of gas is arranged inside the ion guide in such a way that the gas drag counteracts the force exerted by the axial field. Ions with different mobility coefficients can be scanned out of the ion guide by ramping the axial field strength. The ions can be analyzed intact or fragmented in a collision cell before introduction into an orthogonal TOF mass spectrometer. An ion source with matrix assisted laser desorption/ionization (MALDI) was attached to the instrument. The setup was evaluated for the analysis of peptide and protein mixture, with sequential fragmentation of multiple precursor ions from a protein digest and with mobility separation of fragment ions formed by in-source fragmentation of pure peptides. The mobility resolution for peptides was observed to be three times higher than the theoretical resolution predicted for a classical mobility setup with similar operating conditions (pressure, field strength, and length).

References

Dec 1, 1990·Analytical Chemistry·H H HillD G McMinn
Aug 28, 2001·Journal of Mass Spectrometry : JMS·I V ChernushevichB A Thomson
Nov 11, 2003·Rapid Communications in Mass Spectrometry : RCM·A V LobodaI V Chernushevich
Feb 8, 2005·Journal of the American Society for Mass Spectrometry·Brandon T RuotoloDavid H Russell

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Citations

Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Jody C May, David H Russell
Nov 26, 2015·Journal of Chromatography. a·Michael A EwingDavid E Clemmer
Dec 20, 2014·Analytical Chemistry·Jody C May, John A McLean
Jul 18, 2015·International Journal of Mass Spectrometry·Yehia M IbrahimRichard D Smith
Oct 22, 2014·Journal of the American Society for Mass Spectrometry·Karsten MichelmannMelvin A Park
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Apr 9, 2019·Analytical and Bioanalytical Chemistry·Ansgar T KirkStefan Zimmermann
Mar 13, 2013·Human Reproduction·Simon E ChauRosemary J Keogh
May 30, 2019·Analytical Chemistry·Kevin GilesMartin Green
Jul 26, 2007·Chemical Reviews·Justin L P BeneschCarol V Robinson

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