Novel IS6110 insertion sites in the direct repeat locus of Mycobacterium tuberculosis clinical strains from the St. Petersburg area of Russia and evolutionary and epidemiological considerations.

Journal of Clinical Microbiology
Igor MokrousovB Vyshnevskiy

Abstract

A modification of spoligotyping with primers derived from the direct repeat (DR) and IS6110 sequences was used to identify IS6110 insertions in the DR locus of Mycobacterium tuberculosis clinical strains from the St. Petersburg area of Russia. Novel IS6110 insertions were identified: (i) in two epidemiologically unlinked Beijing family strains, an asymmetrical direct insertion in DR37; (ii) in a non-Beijing strain, an asymmetrical insertion in the opposite orientation in DR38; (iii) in another non-Beijing strain, a direct insertion in DR38 and one in the opposite orientation in DR14 (DR numbering is according to standard spoligotyping). Our results strengthen an observation that the DR locus structure is extremely conserved in the Beijing genotype. Asymmetrical insertions prevented detection of the adjacent spacer by standard spoligotyping. This, therefore, should be taken into consideration when similar spoligoprofiles that differ only in signals 37 and 38 are interpreted.

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Citations

Dec 30, 2014·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Horng-Yunn DouIh-Jen Su
Apr 25, 2006·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Inna VitolKristin P Bennett
Mar 3, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·V V NikolayevskyyF A Drobniewski
Jun 10, 2010·PloS One·Solomon GhebremichaelGunilla Källenius
Mar 20, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·R Hernández-PandoD Mata-Espinosa
Oct 26, 2005·Journal of Theoretical Biology·Noel H SmithJohn Maynard Smith
Feb 18, 2011·Journal of Clinical Microbiology·Anita C SchürchDick van Soolingen
Nov 1, 2006·Emerging Infectious Diseases·Vladyslav NikolayevskyyFrancis Drobniewski
May 18, 2006·Emerging Infectious Diseases·James M ColbornDonald J Krogstad
Sep 15, 2004·Journal of Clinical Microbiology·Kristin KremerDick van Soolingen

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