Novel ISCOMs from Quillaja brasiliensis saponins induce mucosal and systemic antibody production, T-cell responses and improved antigen uptake

Vaccine
S P CibulskiF Silveira

Abstract

In the last decades, significant efforts have been dedicated to the search for novel vaccine adjuvants. In this regard, saponins and its formulations as "immunostimulating complexes" (ISCOMs) have shown to be capable of stimulating potent humoral and cellular immune responses, enhanced cytokine production and activation of cytotoxic T cells. The immunological activity of ISCOMs formulated with a saponin fraction extracted from Quillaja brasiliensis (QB-90 fraction) as an alternative to classical ISCOMs based on Quil A(®) (IQA) is presented here. The ISCOMs prepared with QB-90, named IQB-90, typically consist of 40-50 nm, spherical, cage-like particles, built up by QB-90, cholesterol, phospholipids and antigen (ovalbumin, OVA). These nanoparticles were efficiently uptaken in vitro by murine bone marrow-derived dendritic cells. Subcutaneously inoculated IQB-90 induced strong serum antibody responses encompassing specific IgG1 and IgG2a, robust DTH reactions, significant T cell proliferation and increases in Th1 (IFN-γ and IL-2) cytokine responses. Intranasally delivered IQB-90 elicited serum IgG and IgG1, and mucosal IgA responses at distal systemic sites (nasal passages, large intestine and vaginal lumen). These results indicate...Continue Reading

References

Feb 27, 2004·Natural Product Research·C KauffmannG Gosmann
Jun 6, 2006·Micron : the International Research and Review Journal for Microscopy·Julia MyschikThomas Rades
Feb 12, 2009·Vaccine·Hong-Xiang SunYi-Ping Ye
Apr 22, 2009·Immunology and Cell Biology·Eugene MaraskovskyDebbie Drane
May 20, 2009·Vaccine·Hong-Xiang SunYi-Ping Ye
Jun 11, 2009·Drug Discovery Today·Derek T O'Hagan, Ennio De Gregorio
Jan 13, 2010·Vaccine·Ali M HarandiUNKNOWN Working Group convened by EUROPRISE
Jul 3, 2010·Human Vaccines·Andrew McKenzieCharmaine Gittleson
Jul 28, 2010·Biologicals : Journal of the International Association of Biological Standardization·Béatris MastelicPaul-Henri Lambert
Oct 30, 2010·Immunity·Robert L CoffmanRobert A Seder
Apr 12, 2011·Phytotherapy Research : PTR·F SilveiraF Ferreira
Apr 22, 2011·Expert Review of Vaccines·Karin Lövgren BengtssonAlbert Dme Osterhaus
Apr 22, 2011·Expert Review of Vaccines·Govind RagupathiDavid Y Gin
Jul 27, 2011·Vaccine·Jose Rey-LadinoRonald Quinn
Sep 7, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Rino Rappuoli
Jan 15, 2013·Drug Delivery and Translational Research·Mehfuz ZamanIstvan Toth
Jul 16, 2013·Seminars in Immunology·Luis A BritoDerek T O'Hagan
Dec 4, 2013·Vaccine·Liang ZhaoAnton P J Middelberg
Apr 29, 2014·Expert Review of Vaccines·Sergio Rosales-Mendoza, Jorge A Salazar-González
Jul 8, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Luis A Brito, Derek T O'Hagan
Mar 31, 2015·Advanced Drug Delivery Reviews·Christopher J H Davitt, Ed C Lavelle
May 30, 2015·Vaccine·Derek T O'Hagan, Christopher B Fox

❮ Previous
Next ❯

Citations

Apr 5, 2016·Vaccines·Vasso Apostolopoulos
Nov 10, 2016·Vaccines·Kenneth Lundstrom
Feb 2, 2017·Therapeutic Delivery·Nirmal MarasiniIstvan Toth
Jul 16, 2019·Future Medicinal Chemistry·Yve Vs MagedansArthur G Fett-Neto
Mar 23, 2018·Frontiers in Immunology·Blaise Corthésy, Gilles Bioley
Jan 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juliane Deise FleckSimone Gasparin Verza
Apr 4, 2021·Pharmaceutics·Kailash C PetkarImran Y Saleem
Jun 9, 2021·Expert Review of Vaccines·Xinyuan ZhangQinjian Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.