Abstract
Tau is a microtubule-associated protein (MAP) whose transcript undergoes complex regulated splicing in the mammalian nervous system. Our previous work with exon 6 established that tau shows a unique expression pattern and splicing regulation profile, and that it utilizes alternative splice sites in several human tissues. The mRNAs from these splicing events, if translated, would result in truncated tau variants that lack the microtubule-binding domain. In this study, we demonstrate that at least one of these tau variants is present as a stable protein in several tissues. The novel isoform shows a localization distinct from that of canonical tau in SH-SY5Y neuroblastoma cells which stably overexpress it. In both normal and Alzheimer's hippocampus, the novel isoform is found in dentate gyrus granular cells and CA1/CA3 pyramidal cells. However, it does not co-localize with canonical tau but, rather, partly co-localizes with MAP2.
Citations
Jun 24, 2005·Acta Neuropathologica·Berta PuigIsidre Ferrer
Oct 24, 2007·Neurogenetics·Katrin BeyerAurelio Ariza
Dec 30, 2011·Neurogenetics·Katrin BeyerAurelio Ariza
Nov 19, 2009·Biochemistry·Nichole E LapointeLester I Binder
Jul 8, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicholas M KanaanLester I Binder
Oct 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gerardo A MorfiniScott T Brady
Apr 29, 2009·International Journal of Molecular Sciences·Katrin BeyerAurelio Ariza
Jan 12, 2016·Biomolecules·Mahmoud Bukar MainaLouise C Serpell
Jul 14, 2012·Pharmacology & Therapeutics·Diana S HimmelsteinLester I Binder
Mar 23, 2004·Journal of Cellular Biochemistry·Min-Hua LuoAthena Andreadis
May 30, 2009·Cell Motility and the Cytoskeleton·James R Bamburg, George S Bloom
Sep 18, 2008·Journal of Neuroscience Research·Nichole E LaPointeScott T Brady
Dec 6, 2006·Journal of Neurochemistry·Junning WangAthena Andreadis
Aug 6, 2008·Journal of Neurochemistry·Abdelmadjid Belkadi, Patrizia LoPresti
Jul 20, 2011·Journal of Cellular Biochemistry·Yan WangAthena Andreadis
Jan 20, 2005·Gene·Stefan StammHermona Soreq
Sep 7, 2005·Gene·Sze-Wah TseAthena Andreadis
Jul 29, 2011·Neurobiology of Aging·Nicholas M KanaanScott T Brady
Jul 15, 2015·Molecular Neurodegeneration·Marie-Laure Caillet-BoudinBruno Lefebvre
Jun 19, 2013·Neurobiology of Aging·Andrea PristeràNadia Canu
Dec 24, 2004·Biochimica Et Biophysica Acta·Athena Andreadis
Feb 21, 2006·Biochimica Et Biophysica Acta·Olivier LeroyMarie-Laure Caillet-Boudin
Dec 1, 2017·Acta Neuropathologica Communications·Ioannis SotiropoulosLuc Buee
Jun 27, 2019·Progress in Neurobiology·Thomas W RöslerGünter U Höglinger
Dec 8, 2020·Frontiers in Neurology·Sebastian S OakleyLouise C Serpell
Apr 19, 2021·Reviews in the Neurosciences·Michael Bell, Hans Zempel