PMID: 9159181May 27, 1997Paper

Novel isoforms of the beta and gamma subunits of the Xenopus epithelial Na channel provide information about the amiloride binding site and extracellular sodium sensing

Proceedings of the National Academy of Sciences of the United States of America
A PuotiJ D Horisberger

Abstract

We have previously identified three homologous subunits alpha, beta, and gamma of the highly selective amiloride-sensitive Na channel from the Xenopus laevis kidney A6 cell line, which forms a tight epithelium in culture. We report here two novel genes, termed beta2 and gamma2, which share 90 and 92% sequence identity with the previously characterized beta and gamma XENaC, respectively. beta2 and gamma2 transcripts were detected in lung, kidney, and A6 cells grown on porous substrate. The physiological and pharmacological profile of the Na channel expressed after alphabeta2gamma XENaC cRNA injection in Xenopus oocyte did not differ from alphabetagamma XENaC. By contrast, the channel expressed after alphabetagamma2 injection showed: (i) a lower maximal amiloride-sensitive sodium current, (ii) a higher apparent affinity for external sodium and inactivation of the sodium current by high sodium concentrations, and (iii) a lower apparent affinity for amiloride (KI alphabetagamma2; 1.34 microM versus alphabetagamma 0.35 microM). These data indicate that the gamma (and/or gamma2) subunit participates in amiloride binding and the sensing of the extracellular sodium concentration. The close homology between gamma and gamma2 will help to...Continue Reading

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Citations

Jul 31, 2003·Progress in Biophysics and Molecular Biology·Albertino BigianiFrancesca Fieni
Aug 28, 1998·Current Opinion in Cell Biology·J D Horisberger
Sep 15, 1998·Kidney International. Supplement·V ValletB C Rossier
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Oct 8, 2005·American Journal of Respiratory Cell and Molecular Biology·Gail OtulakowskiHugh O'Brodovich
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·S Y ChenD Pearce
Dec 12, 2003·The Journal of Biological Chemistry·Emilija VeljkovicFrançois Verrey
May 14, 2004·American Journal of Physiology. Renal Physiology·Fatemeh FouladkouOlivier Staub
Sep 15, 2004·The Journal of Biological Chemistry·Emilija VeljkovicFrancois Verrey
Apr 12, 2011·American Journal of Physiology. Renal Physiology·Benjamin OberfeldOlivier Staub
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E KamyninaO Staub
Oct 2, 2020·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Edward A MeyerRebecca L Cramp
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Feb 1, 2005·The Biochemical Journal·Raffaella FrancaFrançois Verrey
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Jan 25, 2003·Biochimica Et Biophysica Acta·Mikael SchnizlerWolfgang Clauss
Feb 1, 2000·The American Journal of the Medical Sciences·R Greger

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