Novel keratins identified by quantitative proteomic analysis as the major cytoskeletal proteins of equine (Equus caballus) hoof lamellar tissue

Journal of Animal Science
R A CarterH L Galantino-Homer

Abstract

The dermo-epidermal interface that connects the equine distal phalanx to the cornified hoof wall withstands great biomechanical demands, but is also a region where structural failure often ensues as a result of laminitis. The cytoskeleton in this region maintains cell structure and facilitates intercellular adhesion, making it likely to be involved in laminitis pathogenesis, although it is poorly characterized in the equine hoof lamellae. The objective of the present study was to identify and quantify the cytoskeletal proteins present in the epidermal and dermal lamellae of the equine hoof by proteomic techniques. Protein was extracted from the mid-dorsal epidermal and dermal lamellae from the front feet of 5 Standardbred geldings and 1 Thoroughbred stallion. Mass spectrometry-based spectral counting techniques, PAGE, and immunoblotting were used to identify and quantify cytoskeletal proteins, and indirect immunofluorescence was used for cellular localization of K14 and K124 (where K refers to keratin). Proteins identified by spectral counting analysis included 3 actin microfilament proteins; 30 keratin proteins along with vimentin, desmin, peripherin, internexin, and 2 lamin intermediate filament proteins; and 6 tubulin microt...Continue Reading

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Citations

May 13, 2015·Veterinary Dermatology·Renata L LinardiHannah L Galantino-Homer
Aug 29, 2017·PloS One·Pierre BalmerVidhya Jagannathan
Nov 20, 2019·Toxicologic Pathology·Qingqiu Yang, Mandi J Lopez
Jul 22, 2014·Experimental Dermatology·Robert K Clark, Hannah L Galantino-Homer
Jul 1, 2020·Frontiers in Veterinary Science·Allison CampoloVéronique A Lacombe

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