Novel label-free electrochemical aptasensor for determination of Diazinon using gold nanoparticles-modified screen-printed gold electrode

Biosensors & Bioelectronics
Shokoufeh HassaniMohammad Abdollahi

Abstract

The present study aimed to develop a highly sensitive label-free electrochemical aptasensor for the detection of Diazinon (DZN), as one of the most widespread organophosphorus compounds. The aptasensor was assembled using screen-printed gold electrode modified by thiolated aptamers which were immobilized on gold nanoparticles (Au NPs). Optimum deposition time, in which the highest electrochemical response occurred, was found in 150 s. Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize electrochemical properties of the novel aptasensor. Electrochemical detection was carried out through differential pulse voltammetry in [Fe(CN)6]3-/4- solution. Fluctuation of the current was examined in the DZN concentration range of 0.1-1000 nM. According to the results, the designed aptasensor provided an extremely lower limit of detection (0.0169 nM) compared with HPLC and other colorimetric techniques for DZN detection. The present highly specific designed aptasensor doesn't interact with other analytes in the real sample. Consequently, the present aptasensor is easy to use and relatively inexpensive with a good sensitivity, stability, and reproducibility for this application. We are now evaluating all app...Continue Reading

Citations

Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Shokoufeh HassaniMohammad Abdollahi
Apr 8, 2020·Biosensors·Beatriz Pérez-FernándezAlfredo de la Escosura- Muñiz
Dec 3, 2020·Sensors·Kamonrat Phopin, Tanawut Tantimongcolwat
Feb 26, 2020·Bioelectrochemistry·Hedieh Asadi Samie, Majid Arvand
Feb 12, 2021·International Journal of Nanomedicine·Armin Salek MaghsoudiMohammad Abdollahi
Mar 11, 2021·Environmental Research·Mohammad Reza SepandAmir Shadboorestan
May 13, 2021·Biosensors & Bioelectronics·Lei HuangJinhong Guo
Sep 15, 2020·ACS Applied Materials & Interfaces·Abbas Parvez KaziDionysios C Christodouleas
Oct 12, 2021·Critical Reviews in Biotechnology·Chitra Padmakumari KurupMinhaz Uddin Ahmed
Dec 14, 2021·ACS Omega·Muthaiyan LakshmanakumarJohn Bosco Balaguru Rayappan

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