Novel Liquid Biopsy Test Based on a Sensitive Methylated SEPT9 Assay for Diagnosing Hepatocellular Carcinoma

Hepatology Communications
Yurika KotohTakahiro Yamasaki

Abstract

Liquid biopsies are not used in practice for hepatocellular carcinoma (HCC). Epi proColon is the first commercial blood-based test for colorectal cancer screening based on methylated DNA testing of the septin 9 gene (SEPT9). However, Epi proColon has some disadvantages, including the requirement of a large amount of blood and lack of quantitative performance. Therefore, we previously developed a novel liquid biopsy test that can quantitatively detect even a single copy of methylated SEPT9 in a small amount of DNA. In the current study, we evaluated the application potential of this assay for diagnosing HCC. Study subjects included 80 healthy volunteers, 45 patients with chronic liver disease (CLD) without HCC, and 136 patients with HCC (stage 0, 12; stage A, 50; stage B, 31; stage C, 41; and stage D, 2), according to the Barcelona Clinic Liver Cancer staging system. For the assay, DNA was treated with methylation-sensitive restriction enzymes in two steps, followed by multiplex droplet digital polymerase chain reaction. The median copy number of methylated SEPT9 was 0.0, 2.0, and 6.4 in the healthy control, CLD, and HCC groups, respectively, with significant differences among the groups (HCC vs. healthy control, P < 0.001; HCC ...Continue Reading

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Citations

Jan 10, 2021·International Journal of Molecular Sciences·Vijay PandyarajanJu Dong Yang
May 2, 2021·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·Ayman S BannagaRamesh P Arasaradnam
Sep 18, 2021·Hepatology Communications·Nia Adeniji, Renumathy Dhanasekaran

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Methods Mentioned

BETA
PCR
biopsy
biopsies
immunoprecipitation
surgical resection
Assay

Software Mentioned

proColon
QuantaSoft
Epi
JMP

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