PMID: 11339282May 8, 2001Paper

Novel liquid chromatographic-tandem mass spectrometric methods using silica columns and aqueous-organic mobile phases for quantitative analysis of polar ionic analytes in biological fluids

Journal of Chromatography. B, Biomedical Sciences and Applications
Weng NaidongX Jiang

Abstract

Use of silica stationary phase and aqueous-organic mobile phases could significantly enhance LC-MS-MS method sensitivity. The LC conditions were compatible with MS detection. Analytes with basic functional groups were eluted with acidic mobile phases and detected by MS in the positive ion mode. Analytes with acid functional groups were eluted with mobile phases at neutral pH and detected by MS in the negative ion mode. Analytes poorly retained on reversed-phase columns showed good retention on silica columns. Compared with reversed-phase LC-MS-MS, 5-8-fold sensitivity increases were observed for basic polar ionic compounds when using silica columns and aqueous-organic mobile phase. Up to a 20-fold sensitivity increase was observed for acidic polar ionic compounds. Silica columns and aqueous-organic mobile phases were used for assaying nicotine, cotinine, and albuterol in biological fluids.

References

Jun 1, 1996·Analytical Chemistry·D R DoergeS Bajic

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Citations

Sep 1, 2011·Analytical and Bioanalytical Chemistry·Bogusław Buszewski, Sylwia Noga
Oct 29, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Weng Naidong
Nov 4, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yan DingMichael G Bartlett
Mar 13, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Angela EerkesWeng Naidong
Apr 9, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yu-Luan ChenWeng Naidong
Jun 8, 2006·Experimental and Clinical Psychopharmacology·Sarah E EvansThomas Eissenberg
Nov 30, 2006·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Alison B BrelandThomas Eissenberg
Mar 8, 2008·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Melissa D BlankThomas Eissenberg
Nov 22, 2008·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Jennifer N GrayThomas Eissenberg
Feb 18, 2010·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Melissa D Blank, Thomas Eissenberg
Dec 4, 2010·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Caroline O CobbThomas Eissenberg
Apr 21, 2011·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Melissa D BlankThomas Eissenberg
Jan 18, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Shayna T BradfordAnuska V Andjelkovic
Sep 24, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jennifer A DavisThomas J Gould
Oct 16, 2001·Journal of Pharmaceutical and Biomedical Analysis·W NaidongX Jiang
Mar 9, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rufus TurnerAnthony J Kettle
Dec 15, 2015·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Carolina P RamôaThomas Eissenberg
Jan 31, 2009·Journal of Chromatography. a·Zhengjin JiangNorman W Smith
Jan 16, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pierre N M DemackerJohannes M W van den Ouweland
Jul 29, 2008·Journal of Pharmaceutical and Biomedical Analysis·Juris HmelnickisKaspars Kokums
Apr 24, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yunsheng HsiehMing Liu
Feb 28, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Todd M BaughmanKathryn A Hutton
Jul 17, 2009·Journal of Separation Science·Zhengjin JiangMark R Taylor
Apr 23, 2008·Journal of Separation Science·Yunsheng Hsieh
Apr 11, 2008·Journal of Separation Science·Hien P Nguyen, Kevin A Schug
Apr 10, 2008·Journal of Separation Science·Mohammed Shahid AliAamer Roshanali Khatri
Sep 27, 2014·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Caroline O CobbThomas Eissenberg

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