Novel lncRNA UPLA1 mediates tumorigenesis and prognosis in lung adenocarcinoma.

Cell Death & Disease
Xiaoyang HanChuanxi Wang

Abstract

With the development of molecular biotechnology and sequencing techniques, long non-coding RNAs (lncRNAs) have been shown to play a vital role in a variety of cancers including lung cancer. In our previous study, we used RNA sequencing and high-content screening proliferation screening data to identify lncRNAs that were significantly associated with tumour biological functions such as LINC01426. Herein, based on previous work, we report a novel lncRNA UPLA1 (upregulation promoting LUAD-associated transcript-1), which has not been explored or reported in any previous studies. Our results showed that UPLA1 is highly expressed and regulates important biological functions in lung adenocarcinoma. In vitro experiments revealed that UPLA1 promoted the migration, invasion, and proliferation abilities, and is related to cell cycle arrest, in lung adenocarcinoma cells. Moreover, the upregulation of UPLA1 significantly improved the growth of tumours in vivo. We identified that UPLA1 was mainly located in the nucleus using fluorescence in situ hybridisation, and that it promoted Wnt/β-catenin signalling by binding to desmoplakin using RNA pulldown assay and mass spectrometry. Additionally, luciferase reporter assay revealed that YY1 is the...Continue Reading

References

Sep 20, 2005·Breast Cancer Research : BCR·Marc M OshiroBernard W Futscher
Sep 15, 2007·Biochimica Et Biophysica Acta·David Garrod, Martyn Chidgey
Dec 24, 2010·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Andreas BourdoumisCharalambos Deliveliotis
Mar 19, 2013·Cell·Pedro J Batista, Howard Y Chang
Aug 28, 2014·Biochimica Et Biophysica Acta·Guodong YangLijun Yuan
Jun 3, 2015·Breast Cancer Research and Treatment·Lei ZhangHuiqing Cao
Jun 5, 2016·Surgical Oncology Clinics of North America·Min Zheng
Jul 1, 2014·Molecular & Cellular Oncology·Chunlai LiChunru Lin
Aug 31, 2016·Lancet·Fred R HirschLuis Paz-Ares
Jul 14, 2017·Cancer Research·Arunoday BhanSubhrangsu S Mandal
Apr 21, 2018·Seminars in Cancer Biology·Tianzuo ZhanMichael Boutros
May 6, 2019·Seminars in Cell & Developmental Biology·Itishree KaushikSanjay K Srivastava
Oct 9, 2019·International Journal of Biological Sciences·Peipei LiLi-Jun Di
Dec 12, 2019·Frontiers in Oncology·Sailu SarvagallaSivakumar Vallabhapurapu
Feb 19, 2020·CA: a Cancer Journal for Clinicians·Javier CortesJoaquín Arribas
Jun 1, 2020·Pharmacology & Therapeutics·Qingsong HuLiuqing Yang
Sep 22, 2020·Molecular Therapy. Methods & Clinical Development·Baorui TianChuanxi Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
pulldown
transfection
confocal microscopy
Pull-Down
electrophoresis
flow cytometry
PCR
transfect
in vitro transcription

Software Mentioned

SPSS
Excel
Ensembl
GraphPad Prism
CellQuest Pro

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.