Novel long non-coding RNA LINC02532 promotes gastric cancer cell proliferation, migration, and invasion in vitro

World Journal of Gastrointestinal Oncology
Cheng ZhangDong-Qiu Dai

Abstract

Long non-coding RNAs (lncRNAs) are a kind of single-stranded RNA of more than 200 nucleotides in length and have no protein-coding function. Amounting studies have indicated that lncRNAs could play a vital role in the initiation and development of cancers, including gastric cancer (GC). Considering the crucial functions of lncRNAs, the identification and exploration of novel lncRNAs in GC is necessary. To explore the role of novel lncRNA LINC02532 in GC. The upregulated LINC02532 was identified by processing the GC RNA-Seq data from The Cancer Genome Atlas. The qRT-PCR assay was performed to confirm the expression levels in GC cell lines and tissues. Cell proliferation, migration, and invasion were evaluated by the cell counting kit-8, colony formation, wound healing, and Transwell assays. The miRNAs downregulated in GC and sponged by LINC02532 were identified from and predicted by the data from the Firehose and RNA22 software programs, respectively. The miRNA downstream target genes were obtained from the TargetScan, miRDB, and DIANA online tools. Gene functional enrichment analysis was carried out using the Database for Annotation, Visualization, and Integrated Discovery software in the categories of cellular components, biol...Continue Reading

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Citations

Jul 13, 2019·Journal of Cellular Biochemistry·Min XueXiwen Gao
Oct 24, 2020·Journal of Cellular Physiology·Omkar Suhas Vinchure, Ritu Kulshreshtha
Nov 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoguang ZhouXiaodong Zhang
Dec 11, 2021·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·Qing CaiLing Wang

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Methods Mentioned

BETA
RNA-Seq
PCR
transfection

Software Mentioned

TargetScan
RNA22
DIANA
RNALocate
Database for Annotation ,
SPSS
Database for Annotation , Visualization and Integrated Discovery
miRDB
LncRNA
iLoc

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