Novel macrolide-resistance genes, mef(C) and mph(G), carried by plasmids from Vibrio and Photobacterium isolated from sediment and seawater of a coastal aquaculture site

Letters in Applied Microbiology
Lisa NonakaMichiaki Masuda

Abstract

The aim of this study was to determine whether mef(C) and mph(G), originally found on the transferable multi-drug plasmid pAQU1 from Photobacterium damselae subsp. damselae isolated from seawater of a fish farm, are responsible for conferring macrolide resistance. Since these genes are localized head-to-tail on pAQU1 and only four nucleotides exist between them, the single- and combination-effect of these genes was examined. When mph(G) alone was introduced to Escherichia coli, the minimum inhibitory concentrations (MICs) against erythromycin, clarithromycin and azithromycin increased, whereas introduction of mef(C) alone did not influence macrolide susceptibility. Introduction of both mef(C) and mph(G) dramatically increased the MICs to the same three macrolides, i.e. >512 μg ml(-1) , >512 μg ml(-1) and 128 μg ml(-1) respectively. These results suggest that the macrolide phosphotransferase encoded by mph(G) is essential for macrolide resistance, while the efflux pump encoded by mef(C) is required for high-level macrolide resistance. The tandem-pair arrangements of the mef(C) and mph(G) genes were conserved on plasmids ranging in size from 240 to 350 kb of the 22 erythromycin-resistant strains belonging to Vibrio and Photobacte...Continue Reading

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Citations

Apr 17, 2016·The Lancet Infectious Diseases·Felipe C CabelloHumberto J Dölz
Feb 2, 2018·Frontiers in Microbiology·Juan J González-PlazaNikolina Udiković-Kolić
Jan 11, 2018·Future Microbiology·John Osei Sekyere, Jonathan Asante
Aug 17, 2016·Cold Spring Harbor Perspectives in Medicine·Corey FyfeJoyce Sutcliffe
Jul 1, 2017·British Journal of Pharmacology·George P Dinos
Jan 11, 2018·Nature Communications·Andrew C PawlowskiGerard D Wright
Mar 26, 2021·Environmental Science and Pollution Research International·Sakina BombaywalaSunil Kumar
Jul 24, 2021·Journal of Environmental Management·Sakina BombaywalaNishant A Dafale

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