Novel membrane devices and their potential utility in blood sample collection prior to analysis of dried plasma spots

Bioanalysis
Robert SturmPhil Wang

Abstract

Construction and application of a novel membrane substrate dried plasma spot (DPS) card is described. Results/methodology: The online automation compatible prototype DPS card employs a membrane filter to remove red blood cells from whole blood for the collection of plasma. The described autoDPS card provides acceptable quantitative precision and accuracy data from plasma filtered from 45% hematocrit whole blood. When significantly lower or higher hematocrit (30 and 60%) whole blood fortified with guanfacine is applied, the quantitative precision and accuracy data fall outside regulated 15/20 bioanalytical acceptance criteria. The described prototype DPS card works well for normal hematocrit whole blood, but further development is needed for samples of much lower or higher hematocrit.

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Citations

Apr 19, 2016·Expert Opinion on Drug Metabolism & Toxicology·Suzanne L ParkerJason A Roberts
May 16, 2017·Molecular & Cellular Proteomics : MCP·Johan BjörkestenUlf Landegren
May 21, 2020·British Journal of Clinical Pharmacology·Bradley D MenzStephanie E Reuter
Aug 11, 2020·Bioanalysis·Neil SpoonerEnaksha R Wickremsinhe
Aug 30, 2018·International Journal of Analytical Chemistry·Alexander Chernonosov
Apr 4, 2021·Micromachines·Zaidon T Al-AqbiMohamed T A Soliman
Jan 21, 2021·Therapeutic Drug Monitoring·Lisa DelahayeChristophe Stove
Nov 1, 2018·Analytical Chemistry·Janosch HauserNiclas Roxhed
May 8, 2019·Analytical Chemistry·Janosch HauserNiclas Roxhed

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Methods Mentioned

BETA
red
red blood cell filtration
plasma collection
membrane filtration

Software Mentioned

Exactive Plus
Sparklink
Adobe Illustrator
LCquan
Xcalibur
DBSA
Spark

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