Novel metabolic aspects related to adenosine deaminase inhibition in a human astrocytoma cell line

Neurochemistry International
Mercedes Garcia-GilM Camici

Abstract

Adenosine deaminase, which catalyzes the deamination of adenosine and deoxyadenosine, plays a central role in purine metabolism. Indeed, its deficiency is associated with severe immunodeficiency and abnormalities in the functioning of many organs, including nervous system. We have mimicked an adenosine deaminase-deficient situation by incubating a human astrocytoma cell line in the presence of deoxycoformycin, a strong adenosine deaminase inhibitor, and deoxyadenosine, which accumulates in vivo when the enzyme is deficient, and have monitored the effect of the combination on cell viability, mitochondrial functions, and other metabolic features. Astrocytomas are the most common neoplastic transformations occurring in glial cell types, often characterized by a poor prognosis. Our experimental approach may provide evidence both for the response to a treatment affecting purine metabolism of a tumor reported to be particularly resistant to chemotherapeutic approaches and for the understanding of the molecular basis of neurological manifestations related to errors in purine metabolism. Cells incubated in the presence of the combination, but not those incubated with deoxyadenosine or deoxycoformycin alone, underwent apoptotic death, w...Continue Reading

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Citations

Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Mar 10, 2018·International Journal of Molecular Sciences·Marcella CamiciMaria Grazia Tozzi
Dec 7, 2018·Chemical Society Reviews·Junji ZhangHe Tian
Sep 25, 2019·Cancers·Marcella CamiciMaria Grazia Tozzi
Nov 18, 2018·International Journal of Molecular Sciences·Mercedes Garcia-GilMaria Grazia Tozzi
Sep 15, 2019·International Journal of Biological Macromolecules·S BagheriT Haertlé

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