PMID: 14746552Jan 30, 2004

Novel method for cell immobilization and its application for production of oligosaccharides from sucrose

Letters in Applied Microbiology
H T ShinJ H Lee

Abstract

The purpose of the present investigation was to develop a novel method for cell immobilization. Aureobasidium pullulans cells were mixed with an alginate solution, and the mixture was extruded to form small gel beads as hydrated-immobilized cells. The beads were then placed at -15 degrees C for 6-24 h to induce freeze-dehydration. The freeze-dehydration resulted in shrinkage of beads as a result of water removal reducing bead volume by 82% and bead weight by 85%. The dehydrated beads were successfully used for the production of fructo-oligosaccharides in a model reactor system. Dehydrated beads may provide some commercial advantages over conventional immobilized cells. This study shows that bioreactor performance can be improved up to two times by the use of the dehydrated beads.

Citations

Dec 18, 2013·Applied Biochemistry and Biotechnology·Mohd Anis GanaieUma Shanker Gupta
Nov 17, 2016·Applied Microbiology and Biotechnology·Antonio Irineudo MagalhãesCarlos Ricardo Soccol
Jan 25, 2013·International Journal of Molecular Sciences·Fabiano Jares ContesiniHélia Harumi Sato

Related Concepts

Xantalgin
Fructooligosaccharide
Alginates
Cochliobolus
Cell Adhesion
Water Stress
Enzyme Stability
Fermentation
Freeze Drying
Hexuronic Acids

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.