Novel method for genotyping hepatitis B virus on the basis of TaqMan real-time PCR.

Journal of Clinical Microbiology
Sebastian MalmströmMagnus Lindh

Abstract

Chronic infection with hepatitis B virus (HBV) is an important cause of cirrhosis and cancer of the liver. HBV is currently classified into eight genotypes, A to H. Accumulated evidence shows that the genotype influences both the clinical course of infection and the response to treatment. We describe a new method for genotyping based on TaqMan real-time PCR, which identifies all HBV genotypes without post-PCR processing. In this assay, each sample is processed in four multiplex real-time PCRs, each targeting two or three genotype-specific segments of HBV. By analyzing 185 samples representing all genotypes and different proportions of genotype mixtures, we could validate high accuracy of the assay. We conclude that this new assay represents a significant advancement for both diagnostics and clinical research because it is accurate, practical, and based on a technique that is well established in many virological laboratories.

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Citations

Oct 15, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Simon B LarssonMagnus Lindh
May 23, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sebastian MalmströmMagnus Lindh
Sep 13, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Zhejun CaiNanping Wu
Oct 26, 2016·Nature Reviews. Gastroenterology & Hepatology·Jean-Pierre Allain, Ohene Opare-Sem
Oct 9, 2016·Archives of Virology·Andréia P AndradeAldemir B Oliveira-Filho
Oct 4, 2019·Journal of Laboratory Physicians·Iram MasoodHasan Abbas Zaheer
Oct 31, 2014·World Journal of Gastroenterology : WJG·Sibnarayan DattaVijay Veer
May 31, 2016·World Journal of Gastroenterology : WJG·Mohammad IrshadMohammad Ahmad Ansari

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