Novel method for measuring junctional proton permeation in isolated ventricular myocyte cell pairs

American Journal of Physiology. Heart and Circulatory Physiology
Pawel Swietach, Richard D Vaughan-Jones

Abstract

Partial exposure of single ventricular myocytes to membrane-permeant weak acids or bases, using a dual-microperfusion technique, generates large and stable intracellular pH (pHi) gradients. In this study, we have investigated the feasibility of using the technique to estimate junctional proton permeability. This was done by recording the pHi gradient developed across the junctional region of a pair of conjoined ventricular myocytes, isolated enzymically from a guinea pig heart when one of the cells was partially exposed to acetate or ammonium. We show that under HEPES-buffered conditions, the junctional discontinuity in the pHi profile can be used to derive an apparent proton permeability coefficient (PHapp). The mean PHapp obtained was 4.45 +/- 0.21.10(-4) cm/s (n=43) at an average junctional pHi of 7.04 +/- 0.02. In the presence of the junctional inhibitor alpha-glycyrrhetinic acid, exposure of the proximal cell to weak acid or base produced no pHi change in the distal cell, confirming that distal changes were normally caused by acid-base flux through connexons assembled into junctional channels. The validity of the dual-microperfusion method was tested further by using a diffusion-permeation-reaction model for intracellular ...Continue Reading

References

Aug 1, 1976·The Journal of Physiology·R W Tsien, R Weingart
Apr 1, 1990·The American Journal of Physiology·O RimoldiA Malliani
Nov 1, 1989·The Journal of Physiology·C Bountra, R D Vaughan-Jones
Feb 1, 1989·The American Journal of Physiology·K W ScheelS E Williams
Jan 16, 1987·Biochimica Et Biophysica Acta·W Junge, S McLaughlin
Feb 1, 1987·Circulation Research·D G Allen, C H Orchard
Jan 1, 1987·The American Journal of Physiology·I ImanagaH Irisawa
Jan 1, 1974·The Journal of Membrane Biology·I Imanaga
Oct 1, 1981·Physiological Reviews·W R Loewenstein
Sep 1, 1983·Journal of Molecular and Cellular Cardiology·W C De MelloP van Loon
Apr 1, 1981·Physiological Reviews·A Roos, W F Boron
Sep 1, 1994·Cardiovascular Research·C H Orchard, H E Cingolani
Jun 25, 1998·The American Journal of Physiology·C J HyattW E Cascio
May 5, 1999·The Journal of Physiology·C H LeemR D Vaughan-Jones
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·K W SpitzerR D Vaughan-Jones
Apr 7, 2000·Brain Research. Brain Research Reviews·R RozentalM F Mehler
Jan 16, 2002·The Journal of Physiology·Christof J Schwiening, Debbie Willoughby
May 17, 2002·The Journal of Physiology·R D Vaughan-JonesK W Spitzer
Jul 27, 2002·Circulation Research·Virginijus ValiunasPeter R Brink
May 17, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Massimiliano ZaniboniRichard D Vaughan-Jones
Jul 17, 2003·Progress in Biophysics and Molecular Biology·Pawel SwietachRichard D Vaughan-Jones
Sep 6, 2003·Circulation Research·Massimiliano ZaniboniRichard D Vaughan-Jones
Sep 23, 2003·Circulation Research·Jian-An YaoAndrew L Wit
Nov 14, 2003·Cardiovascular Research·Joris R de GrootE Etienne Verheijck

❮ Previous
Next ❯

Citations

Jan 24, 2019·Cancers·Pawel Swietach, Stefania Monterisi
May 2, 2007·Progress in Biophysics and Molecular Biology·Andrew L Harris

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Physiology
Richard D Vaughan-JonesKenneth W Spitzer
Circulation Research
Massimiliano ZaniboniRichard D Vaughan-Jones
Annals of the New York Academy of Sciences
Pawel Swietach, Richard D Vaughan-Jones
© 2022 Meta ULC. All rights reserved