Novel method for the depletion of cariogenic bacteria using dextranomer microspheres

Molecular Oral Microbiology
Lauren Mashburn-WarrenSteven D Goodman

Abstract

Streptococcus mutans is recognized as one of the key contributors to the dysbiotic state that results in dental caries. Existing treatment strategies reduce the incidence of tooth decay, but they also eliminate both the cariogenic and beneficial microbes. Here we introduce a novel treatment alternative using Sephadex, cross-linked dextranomer microspheres (DMs), typically used for gel filtration chromatography. In addition DM beads can be used for affinity purification of glucosyltransferases (GTFs) from S. mutans. In this study we take advantage of the native pathogenic mechanisms used by S. mutans to adhere, form a biofilm and induce dental caries through the expression of surface-associated GTFs. We demonstrate that planktonic and biofilm-grown (adhered to hydroxyapatite-coated pegs to mimic the tooth surface) S. mutans, specifically and competitively attach to DMs. Further investigation demonstrated that DMs are a specific affinity resin for S. mutans and other cariogenic/pathogenic oral streptococci, whereas other commensal and probiotic strains failed to readily adhere to DMs. Using antimicrobial cargo loaded into the DM lumen, we demonstrate that when in co-culture with non-binding to even modestly binding commensal spec...Continue Reading

References

Jun 1, 1978·Infection and Immunity·H K Kuramitsu, L Ingersoll
Feb 1, 1977·Chest·F R Sattler, J Ruskin
Apr 1, 1977·British Heart Journal·D McGhieA P Ball
May 1, 1977·Irish Journal of Medical Science·J MooreG H Tomkin
Mar 23, 1987·The American Journal of Medicine·J M VoseD Colan
Mar 1, 1974·Annals of Internal Medicine·W R LockwoodF S Morrison
Jan 1, 1983·Methods in Enzymology·M RosenbergA Shatzman
Feb 1, 1980·Infection and Immunity·I van de Rijn, R E Kessler
May 1, 1995·Journal of Dental Research·A PrakobpholS J Fisher
Sep 1, 1994·The American Journal of Medicine·P Y BochudP Francioli
Jul 8, 1998·European Journal of Biochemistry·K D GrasserJ O Thomas
May 26, 1999·The Journal of Infection·C DysonG A Harrison
Dec 29, 1999·FEMS Microbiology Letters·D G CvitkovitchA S Bleiweis
Mar 10, 2001·American Journal of Public Health·UNKNOWN American Public Health Association
Apr 4, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R MarkovskaE Keuleyan
Jul 18, 2002·Oral Microbiology and Immunology·Y SatoH Kizaki
May 24, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J A Banas, M M Vickerman
Mar 24, 2004·Nature Reviews. Microbiology·Timothy J Mitchell
Sep 24, 2005·Infection and Immunity·Saswati Biswas, Indranil Biswas
Nov 8, 2005·Journal of Clinical Microbiology·Jørn A AasFloyd E Dewhirst
Mar 11, 2009·Archives of Internal Medicine·David R MurdochUNKNOWN International Collaboration on Endocarditis-Prospective Cohort Study (ICE-PCS) Investigators
Sep 3, 2009·Future Microbiology·Kazuhiko Nakano, Takashi Ooshima
Nov 18, 2009·Journal of Dental Research·S FilocheC H Sissons
Oct 26, 2010·Molecular Microbiology·Lauren Mashburn-WarrenMichael J Federle

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Related Papers

Refuʾat ha-peh ṿeha-shinayim : iṭʾon ha-Histadrut li-refuʾat shinayim be-Yiśraʾel = the journal of the Israel Dental Association
Benny Peretz
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology
W X Yu
© 2022 Meta ULC. All rights reserved