Novel methods for identification and quantification of the mushroom nephrotoxin orellanine. Thin-layer chromatography and electrophoresis screening of mushrooms with electron spin resonance determination of the toxin

Journal of Chromatography. a
H OubrahimF Trécourt

Abstract

Orellanine, (2,2'-bipyridine)-3,3',4,4'-tetrol-1,1'-dioxide, the toxin from several Cortinariace species, induces an acute renal failure which can be very severe or even irreversible and fatal. It is therefore important to be able to quickly and simply identify orellanine in mushroom samples with classical methods, readily available in any laboratory, such as anti-poison centers. This article reports the results of three analytical methods: classical TLC on cellulose plates in n-butanol--acetic acid--water and two original methods, electrophoresis on agarose gel and direct electron spin resonance (ESR) after enzymatic oxidation. They were applied to detect orellanine in 34 Cortinariaceae and 4 other species of toadstools. Our three sets of results are convergent. TLC (detection limit: 15 ng with fluorescence densitometry), electrophoresis (25 ng) and even ESR (5 micrograms), are sensitive enough for our purpose, and a sophisticated method like HPLC (detection limit: 50 pg) is not required. As the ESR spectrum of the toxin semiquinone is highly specific, TLC or electrophoresis coupled with ESR are a convenient alternative to liquid chromatography coupled with mass spectrometry, with the same specificity, for a confirmation or wi...Continue Reading

Citations

Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·V C DanelD Garon
Sep 25, 2003·Toxicon : Official Journal of the International Society on Toxinology·Christine Karlson-Stiber, Hans Persson
Oct 11, 2012·Journal of Natural Products·Anders HerrmannKarl-Erik Hellenäs
Apr 16, 2002·Internal Medicine Journal·P MountG J Becker
Mar 3, 2006·Der Internist·H FischerJ Mann
Feb 27, 2016·Toxicon : Official Journal of the International Society on Toxinology·Dahai ShaoWilson K Rumbeiha
Oct 26, 2012·QJM : Monthly Journal of the Association of Physicians·P NagarajaM Kumwenda
Nov 11, 2015·Human & Experimental Toxicology·Ricardo Jorge Dinis-OliveiraFélix Carvalho
May 21, 2005·Therapeutic Drug Monitoring·Vanessa Steenkamp, Michael J Stewart
Aug 22, 2018·Toxins·Deman NajarKerstin Ebefors
Jul 13, 2000·Journal of Chromatography. a·J Sherma

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