Novel methods of genome shuffling in Saccharomyces cerevisiae

Biotechnology Letters
Lihua Hou

Abstract

Genome shuffling can improve complex phenotypes; however, there are several obstacles towards its broader applicability due to increased complexity of eukaryotic cells. Here, we describe novel, efficient and reliable methods for genome shuffling to increase ethanol production of Saccharomyces cerevisiae. Using yeast sexual and asexual reproduction by itself, mutant diploid cells were shuffled through highly efficient sporulation and adequate cross among the haploid cells, followed by selection on the special plates. The selected strain obtained after three round genome shuffling not only distinctly improved the resistance to ethanol, but also, increased ethanol yield by up to 13% compared with the control.

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Citations

Jul 24, 2010·Journal of Industrial Microbiology & Biotechnology·Dao-Qiong ZhengYu-Hua Zhao
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Xiaohong CaoLihua Hou
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Pu ZhengZhi-Hao Sun
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Aug 5, 2018·Journal of Industrial Microbiology & Biotechnology·Xin XuQi Li
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Sep 25, 2020·World Journal of Microbiology & Biotechnology·Liang ChenKe Bian
Nov 7, 2020·Frontiers in Bioengineering and Biotechnology·Eugene Fletcher, Kristin Baetz

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