Novel methods to determine the epitopes on the asparagine-linked oligosaccharides of glycoproteins.

Analytical Biochemistry
H OhbayashiA Kobata

Abstract

Oligosaccharides obtained from glycoproteins by hydrazinolysis followed by N-acetylation were covalently coupled to an amino-bonded thin-layer plate or to a poly-L-lysine coating on the microtiter plate. Bound oligosaccharides can be directly detected by immunostaining or by enzyme-linked immunosorbent assay (ELISA) using a mouse monoclonal antibody. These methods are simple and require small amounts of oligosaccharides and antibodies. The methods were successfully applied to determine the epitope of F48-60, a monoclonal antibody which reacts with the N-linked sugar chains of nonspecific cross-reacting antigen 2, but not with those of carcinoembryonic antigen. The results revealed that the antibody recognizes the Gal beta 1----3GlcNAc beta 1----group.

References

Oct 1, 1985·Analytical Biochemistry·J L Magnani
Dec 15, 1986·Chemistry and Physics of Lipids·J L Magnani
Feb 12, 1986·Biochimica Et Biophysica Acta·P FredmanL Svennerholm

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Citations

Oct 5, 2010·Stem Cells and Development·Mohamed G ShoreibahJeffrey C Gildersleeve
Feb 6, 1999·The Journal of Biological Chemistry·M Tandai-HirumaA Kobata
Mar 4, 2000·Endocrine Reviews·M P RoseA H Balen
Nov 1, 1994·Phytochemistry·L Wan, R B van Huystee

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