PMID: 2505839Jun 27, 1989Paper

Novel methyl transfer during chemotaxis in Bacillus subtilis

Biochemistry
M S ThoelkeG W Ordal

Abstract

If Bacillus subtilis is incubated in radioactive methionine in the absence of protein synthesis, the methyl-accepting chemotaxis proteins (MCPs) become radioactively methylated. If the bacteria are further incubated in excess nonradioactive methionine ("cold-chased") and then given the attractant aspartate, the MCPs lose about half of their radioactivity due to turnover, in which lower specific activity methyl groups from S-adenosylmethionine (AdoMet) replace higher specific activity ones. Due to the cold-chase, the specific activity of the AdoMet pool is reduced at least 2-fold. If, later, the attractant is removed, higher specific activity methyl groups return to the MCPs. Thus, there must exist an unidentified methyl carrier that can "reversibly" receive methyl groups from the MCPs. In a similar experiment, labeled cells were transferred to a flow cell and exposed to addition and removal of attractant and of repellent. All four kinds of stimuli were found to cause methanol production. Bacteria with maximally labeled MCPs were exposed to many cycles of addition and removal of attractant; the maximum amount of radioactive methanol was evolved on the third, not the first, cycle. This result suggests that there is a precursor-pr...Continue Reading

References

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Citations

Jan 1, 1995·Pharmacology & Therapeutics·L F Garrity, G W Ordal
Jun 1, 1994·Research in Microbiology·P B CarpenterG W Ordal
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·George H Wadhams, Judith P Armitage
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E N SpudichJ L Spudich
Jan 1, 1992·Molecular Microbiology·D S Bischoff, G W Ordal
Jun 22, 2006·Journal of Bacteriology·Bonnie B StephensGladys Alexandre
Nov 22, 2001·Journal of Bacteriology·A C MartinJ P Armitage
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Hendrik Szurmant, George W Ordal
Sep 9, 2008·Trends in Microbiology·Christopher V RaoGeorge W Ordal
Jul 28, 2005·Molecular Microbiology·Allison C LamannaLaura L Kiessling
Sep 3, 1998·Biophysical Journal·G CercignaniD Petracchi
Sep 7, 1993·Biochemistry·D S BischoffG W Ordal
Apr 10, 1999·The Journal of Biological Chemistry·J R KirbyG W Ordal
Feb 1, 1990·Journal of Bacteriology·M S ThoelkeG W Ordal
Jul 1, 1995·Journal of Bacteriology·L S WongB L Taylor

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