PMID: 6170064Jul 1, 1981Paper

Novel models for RNA splicing that involve a small nuclear RNA

Proceedings of the National Academy of Sciences of the United States of America
Y OhshimaT Miyata

Abstract

Nucleotide sequences of mammalian small nuclear RNAs (snRNAs) have been analyzed with a computer program for complementarity with sequences around a splice junction of various eukaryotic mRNA precursors (pre-mRNAs). A region in U2 RNA or some other snRNAs can form base pairs with both exons surrounding an intron of certain pre-mRNAs and, thereby, can align the two junctions leading to correct splicing of the pre-mRNA. These findings suggest that a snRNA such as U2 can be involved in splicing certain pre-mRNAs by pairing with exons, which we we call an "exon model" for splicing, as compared with the model involving U1 RNA presented by Lerner et al. [Lerner, M. R., Boyle, J. A., Mount, S. M., Wolin, S. L. & Steitz, J. A. (1980) Nature (London) 283, 220-224]. We constructed a secondary structure model of U1 RNA and studied the capacity of base pairing with pre-mRNAs on the basis of both primary and secondary structures of U1 RNA. We present an alternative model for splicing that involves U1 RNA, which assumes base pairing of noncontiguous regions of U1 RNA with an intron of a pre-mRNA. Pairing of an snRNA with exons could explain correct matching of the two junctions that bound one and the same intron, which is not explained by pa...Continue Reading

References

May 5, 1978·Science·V B ReddyS M Weissman
Dec 25, 1978·Journal of Molecular Biology·P K GhoshS M Weissman
Oct 1, 1979·FEBS Letters·V Murray, R Holliday
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla
Jan 1, 1973·Annual Review of Biochemistry·R A Weinberg
Sep 25, 1980·Nucleic Acids Research·C BranlantP Jeanteur
Mar 6, 1980·Nature·G I BellH M Goodman
Aug 14, 1980·Biochemical and Biophysical Research Communications·F HaradaS Nishimura
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C ReymondG Spohr
Jan 10, 1980·Nature·M R LernerJ A Steitz
Oct 1, 1980·Cell·R A SpritzS M Weissman

❮ Previous
Next ❯

Citations

May 1, 1989·Journal of Steroid Biochemistry·G P RossiniJ A Gustafsson
Mar 1, 1983·Immunology Letters·D W HoughP J Maddison
Jul 1, 1988·Gene Analysis Techniques·R H GrossM S Cetron
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H EsumiT Sugimura
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G WestinU Pettersson
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K TateiY Ohshima
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R SperlingJ Sperling
Jul 1, 1983·The Journal of Cell Biology·D J ForbesM W Kirschner
Jul 24, 1982·Nucleic Acids Research·A BalmainG D Birnie
Mar 25, 1983·Nucleic Acids Research·N Watanabe-NagasuY Ohshima
Dec 21, 1984·Nucleic Acids Research·A AlonsoB Hovemann
Feb 10, 1984·Nucleic Acids Research·M H LiuH Busch
Jul 25, 1985·Nucleic Acids Research·K NakataC DeLisi
Feb 11, 1986·Nucleic Acids Research·J C NobleJ L Manley
Mar 11, 1987·Nucleic Acids Research·N D EpsteinA W Nienhuis
Dec 11, 1981·Nucleic Acids Research·G WestinU Pettersson
Aug 14, 2002·Mayo Clinic Proceedings·Ayalew TefferiThomas C Spelsberg
Dec 25, 1984·Journal of Molecular Biology·A AlonsoT Schmidt
Dec 25, 1984·Journal of Molecular Biology·E MyslinskiT Pederson
Apr 14, 1982·Biochemical and Biophysical Research Communications·B Vogelstein, B F Hunt
Oct 5, 1987·Journal of Molecular Biology·J P Calvet, J A Myers
Dec 1, 1983·Cell·D TollerveyC Guthrie
Sep 25, 1983·Journal of Molecular Biology·A AlonsoE Spiess

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.