Novel molecularly imprinted monolithic column for selective on-line extraction of ciprofloxacin from human urine

Biomedical Chromatography : BMC
Hongyuan Yan, Kyung Ho Row

Abstract

Water-compatible ciprofloxacin-imprinted monolithic columns were synthesized in water-containing systems for selective extraction of ciprofloxacin from human urine samples. Methanol-water (10:3, v/v) was used as a porogenic solvent and the obtained monolithic imprinted polymers reveal high selectivity to ciprofloxacin in an aqueous environment; the affinity can be easily controlled by adjusting the pH of the mobile phase. Owing to the unique porous structure and flow-through channels existing in the network skeleton of the monolithic MIP, urine samples could be directly injected into the column, proteins and other biological matrix were quickly washed out and ciprofloxacin was selectively retained and enriched. Good linearity was obtained from 0.08 to 400 mg/L (r=0.998) with the relative standard deviations less than 3.6%. The limit of detection of the method was 0.04 mg/L and the recoveries were more than 94.5% at three different concentrations. Moreover, by increasing the injection volume to 2.0 mL, the sensitivity of the method could be improved 100-fold according to the peak height of ciprofloxacin. This expedient greatly simplified the overall procedure, resulting in a rapid and efficient sample analysis while maintaining ...Continue Reading

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Citations

Jan 18, 2013·Analytical and Bioanalytical Chemistry·Chao ZhengZhao-Sheng Liu
Feb 23, 2013·Analytical and Bioanalytical Chemistry·Meenakshi SinghNazia Tarannum
Apr 5, 2014·Journal of Molecular Recognition : JMR·Michael J WhitcombeIan A Nicholls
Jul 20, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·E G VlakhT B Tennikova
Sep 8, 2017·Electrophoresis·Mariia А StepanovaTatiana B Tennikova
Aug 17, 2018·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Mahdieh Sadat MohsenzadehSeyed Ahmad Mohajeri
May 11, 2011·Journal of Separation Science·Chao ZhengZhao-Sheng Liu
Jul 28, 2011·Journal of Separation Science·Jens Sproß, Andrea Sinz
Jun 3, 2015·Journal of Separation Science·E G VlakhT B Tennikova

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