Novel mouse IgG-like immunoreactivity expressed by neurons in the moth Manduca sexta: developmental regulation and colocalization with crustacean cardioactive peptide

Microscopy Research and Technique
K A KlukasK A Mesce

Abstract

Immunoglobulin-related molecules have been shown to play important roles in cell-cell recognition events during the development of both vertebrate and invertebrate nervous systems. In the moth, Manduca sexta, we report the presence of novel, mouse, immunoglobulin G (mIgG)-like immunoreactivity in a discrete population of identified neurosecretory neurons (the NS-Ls also known as the cell 27s) and interneurons (the IN-704s). A number of polyclonal anti-mIgG antibodies were used to immunostain these cells in wholemount. The mIgG-like-immunoreactive (IR) neurons were present during embryogenesis through the developing adult stages, but disappeared in the postemerged adult. Biochemical analysis of M. sexta ventral nerve cords revealed that the mIgG-like antigen is a membrane-associated 27-kDa protein which is likely responsible for the mIgG-like immunostaining observed. Unambiguous identification of the mIgG-like-IR neurons was based on neuronal morphology and our ability to demonstrate conclusively that these neurons expressed immunoreactivity to an antiserum against crustacean cardioactive peptide (CCAP). The NS-Ls and IN-704s were both shown to colocalize the CCAP and mIgG-like immunoreactivities. The mIgG-like and CCAP-IR neuro...Continue Reading

References

Oct 1, 1992·The Journal of Cell Biology·R A MeyerR G Johnson
Nov 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E M van der BeekV M Wiegant
Jun 1, 1991·Trends in Neurosciences·N TublitzA W Sylwester
Jan 1, 1991·Annual Review of Neuroscience·L F Reichardt, K J Tomaselli
Jul 15, 1991·The Journal of Comparative Neurology·J B Wall, P H Taghert
Jan 1, 1991·Annual Review of Neuroscience·E A ArbasS R Shaw
Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·G GrenninglohC S Goodman
Jul 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J R TusonD A Jacob
Jul 15, 1990·The Journal of Comparative Neurology·O Breidbach, W Kutsch
Nov 1, 1988·Trends in Neurosciences·H Anderson
Nov 1, 1989·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·B Hille
Jul 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N T BrodinH O Sjögren
Jan 1, 1988·Annual Review of Immunology·A F Williams, A N Barclay
May 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger, N H Sternberger
Jun 1, 1983·Pathology, Research and Practice·M F Rousseau-MerckC Nezelof
Jun 1, 1983·The Journal of Comparative Neurology·J W Truman
Jan 13, 1994·Nature·D Hultmark
Jan 1, 1994·Annual Review of Neuroscience·J S Eisen
Jul 1, 1993·Peptides·H K LehmanJ G Hildebrand
Dec 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·R L HillH E Lebovitz
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J StangierR Keller
Jan 1, 1987·Immunology Today·A F Williams

❮ Previous
Next ❯

Citations

Jul 25, 2000·The Journal of Comparative Neurology·H K LehmanK A Mesce
Dec 26, 2001·The Journal of Comparative Neurology·Kevin M CrispKaren A Mesce
Feb 13, 2001·The Journal of Comparative Neurology·K A MesceK A Klukas
Apr 13, 2002·Frontiers in Neuroendocrinology·Karen A Mesce, Susan E Fahrbach
Nov 26, 1999·Peptides·R NicholsE Zornik

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.