Novel mouse model to study T cell-dependent IgA induction in vivo

Journal of Immunological Methods
Caroline WinsauerAndrey Kruglov

Abstract

Commensal microbiota at the mucosal surfaces controls multiple aspects of body homeostasis. Therefore, regulation of microflora composition by the host is crucial, and one of the mechanisms driving microbiota diversity is the production of large quantities of immunoglobulin A (IgA) at the mucosal surfaces. However, mechanisms of IgA induction in the gut are not completely understood. Here we further characterize a mouse model for studying T cell-dependent IgA production in the gut due to specific genetic ablation of LTβ in RORγt+ cells. Using in utero blockade of the mesenteric lymph node development, we showed that IgA induction in these mice occurs directly in the LP. Furthermore, T cell-dependent IgA inducing mechanism in these mice generates distinct IgA plasma cells producing commensal microflora-binding IgA antibodies. Thus, this model represents a unique in vivo tool for the analysis of T cell-dependent IgA plasma cell generation and their antibody specificity.

References

Feb 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Keiichiro SuzukiSidonia Fagarasan
Nov 18, 2006·Science·J Rodrigo MoraUlrich H von Andrian
Mar 3, 2010·Annual Review of Immunology·Sidonia FagarasanKeiichiro Suzuki
Jan 12, 2011·Annual Review of Immunology·Andrea CeruttiAlejo Chorny
Jan 10, 2012·Annual Review of Immunology·Kenya Honda, Dan R Littman
Jan 18, 2012·The Journal of Experimental Medicine·Cornelia LindnerOliver Pabst

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Citations

Jul 1, 2021·Proceedings of the National Academy of Sciences of the United States of America·Mingzhu ZhengJinfang Zhu

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