Novel multi-depth microfluidic chip for single cell analysis

Journal of Chromatography. a
Sun Yue, Yin Xue-Feng

Abstract

A novel multi-depth microfluidic chip was fabricated on glass substrate by use of conventional lithography and three-step etching technology. The sampling channel on the microchip was 37 microm deep, while the separation channel was 12 microm deep. A 1mm long weir was constructed in the separation channel, 300 microm down the channel crossing. The channel at the weir section was 6 microm deep. By using the multi-depth microfluidic chip, human carcinoma cells, which easily aggregate, settle and adhere to the surface of the channel, can be driven from the sample reservoir to the sample waste reservoir by hydrostatic pressure generated by the difference of liquid level between sample and sample waste reservoirs. Single cell loading into the separation channel was achieved by applying a set of pinching potentials at the four reservoirs. The loaded cell was stopped by the weir and precisely positioned within the separation channel. The trapped cell was lysed by sodium dodecyl sulfate (SDS) containing buffer solution in 20s. This approach reduced the lysing time and improved the reproducibility of chip-based electrophoresis separations. Reduced glutathione (GSH) and reactive oxygen species (ROS) were used as model intracellular compo...Continue Reading

References

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Nov 3, 2005·Journal of Toxicology and Environmental Health. Part a·Wonsuk Yang, Evelyn Tiffany-Castiglioni

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Citations

Jan 30, 2008·Analytical and Bioanalytical Chemistry·Daniel A AteyaFrances S Ligler
Feb 18, 2011·Lab on a Chip·K Scott PhillipsNancy L Allbritton
Aug 7, 2008·Journal of the Royal Society, Interface·Tzu-Chiao Chao, Alexandra Ros
Jan 8, 2010·International Journal of Molecular Sciences·Ming NiHanry Yu
Aug 21, 2007·Journal of Biotechnology·Dayu LiuBingcheng Lin
Jan 9, 2013·Luminescence : the Journal of Biological and Chemical Luminescence·Chunxiu Xu, Longfei Cai
May 20, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Minglu HaoMingyang Jing
Mar 2, 2016·Annual Review of Biomedical Engineering·Tania KonryNoa Cohen
Jul 4, 2018·The Analyst·Kathy RodogiannisMichelle L Kovarik
Mar 29, 2007·Lab on a Chip·Christopher E Sims, Nancy L Allbritton
Nov 30, 2007·Electrophoresis·Václav Kasicka
Jan 1, 2008·Microfluidics and Nanofluidics·Hamish C Hunt, James S Wilkinson
Aug 17, 2014·Talanta·Sébastien MéanceAnne-Marie Haghiri-Gosnet
May 24, 2008·Analytical Chemistry·Jonathan WestAndreas Manz
Mar 3, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Shupeng SheChangyou Gao
Apr 18, 2013·Analytical Chemistry·Michelle L KovarikNancy L Allbritton

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