Novel Multiplex Immunoassays for Quantification of IgG against Group B Streptococcus Capsular Polysaccharides in Human Sera

MSphere
Giada BuffiImmaculada Margarit

Abstract

Group B Streptococcus (GBS) infections constitute a major cause of invasive disease during the first three months of life and an unmet medical need that could be addressed by maternal vaccination. The GBS capsular polysaccharides (CPSs) have shown promise as vaccine targets in clinical studies. A highly specific serological assay to quantify maternal and neonatal anti-CPS antibody levels will be instrumental for GBS vaccine licensure. Here, we describe the development and comparison of two novel multiplex immunoassays (MIAs) based on the Luminex technology for the quantification of IgG antibodies recognizing the five most frequent GBS capsular variants (Ia, Ib, II, III, and V) out of the ten types identified. The first assay is based on the use of biotinylated CPSs coupled to streptavidin-derivatized magnetic microspheres (Biotin-CPS MIA), while the second is a sandwich assay with plain CPSs coupled to magnetic microspheres coated with polysaccharide-specific mouse monoclonal antibodies (Sandwich MIA). Both assays showed good specificity, linearity, and precision, although the Biotin-CPS MIA presented higher sensitivity and lower complexity than the Sandwich MIA. A panel of human sera representing a wide range of anti-CPS IgG c...Continue Reading

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Citations

Aug 9, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Geert Leroux-RoelsOuzama Henry
May 20, 2020·Infection and Drug Resistance·Clara Carreras-AbadKirsty Le Doare

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
ELISA
ELISAs
NMR

Software Mentioned

SAS Enterprise Guide
Plex Manager
Xponent
GASTON
Bio
FlexMAP3D

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