Novel murine model for delayed wound healing using a biological wound dressing with Pseudomonas aeruginosa biofilms

Microbial Pathogenesis
Kenneth S BrandenburgJonathan McAnulty

Abstract

Bacterial biofilms impair healing in 60% of chronic skin wounds. Various animal models (mice, rats, rabbits, and pigs) have been developed to replicate biofilm infected wounds in vivo. We developed a sustained wound infection model by applying preformed Pseudomonas aeruginosa biofilms on a wound dressing to full-thickness murine skin wounds. We bathed a commercially available wound dressing in P. aeruginosa for 48 h, allowing a biofilm to establish on the dressing prior to application to the wound. Dressings were removed from the wounds after 3 days at which time the wound beds contained ∼108 bacterial cells per gram tissue. Significant numbers of P. aeruginosa persisted within the skin wounds for up to 21 days. Un-inoculated wounds reached closure between 9 and 12 days. In contrast, biofilm-inoculated wounds achieved closure between 18 and 21 days. Histologic analysis confirmed decreased re-epithelialization and collagen deposition, coupled with increased inflammation, in the biofilm-inoculated wounds compared to un-inoculated controls. This novel model of delayed healing and persistent infection of full-thickness murine skin wounds may provide a robust in vivo system in which to test novel treatments to prevent wound infectio...Continue Reading

Citations

Apr 27, 2021·Frontiers in Immunology·Zoya VerseyEdana Cassol
Jun 17, 2021·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Alexander G BobrovVlado Antonic
Aug 21, 2021·Future Microbiology·Anjali WarrierThokur Sreepathy Murali

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Related Papers

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Kenneth S BrandenburgCharles J Czuprynski
Biulleten' eksperimental'noĭ biologii i meditsiny
Iu V Ivanov
Polski tygodnik lekarski
A B WALLACE
© 2022 Meta ULC. All rights reserved