Novel mutation breeding method for Streptomyces avermitilis using an atmospheric pressure glow discharge plasma.

Journal of Applied Microbiology
L-Y WangC-Y Bao

Abstract

Avermectins are major antiparasitic agents used commercially in animal health, agriculture and human infections. To improve the fermentation efficiency of avermectins, for the first time a plasma jet generated by a novel atmospheric pressure glow discharge (APGD) was employed to generate mutations in Streptomyces avermitilis. The APGD plasma jet, driven by a radio frequency (RF) power supply with water-cooled and bare-metallic electrodes, was used as a new mutation method to treat the spores of S. avermitilis. The plasma jet yielded high total (over 30%) and positive (about 21%) mutation rates on S. avermitilis, and a mutated strain, designated as G1-1 with high productivity of avermectin B1a and genetic stability, was obtained. Because of the low jet temperature, the high concentrations of the chemically reactive species and the flexibility of its operation, the RF APGD plasma jet has a strong mutagenic effect on S. avermitilis. This is a proof-of-concept study for the use of an RF APGD plasma jet for inducing mutations in microbes. We have shown that the RF APGD plasma jet could be developed as a promising and convenient mutation tool for the fermentation industry and for use in biotechnology research.

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Citations

Aug 26, 2014·Jundishapur Journal of Microbiology·Samia SiddiqueFahim Ashraf Qureshi
May 21, 2014·Applied Microbiology and Biotechnology·Jan-Wilm Lackmann, Julia Elisabeth Bandow
Apr 29, 2014·Applied Microbiology and Biotechnology·Xue ZhangCheng-Yu Bao
Feb 3, 2015·International Journal of Molecular Sciences·Krishna Priya ArjunanSylwia Ptasinska
Dec 21, 2013·Acta Odontologica Scandinavica·Jae-Hoon KimByeong-Hoon Cho
Jul 20, 2018·International Journal of Molecular Sciences·Hongbin WangChengbin Chen
Mar 4, 2020·Preparative Biochemistry & Biotechnology·Zhong-Ce HuYu-Guo Zheng
Mar 29, 2011·Prikladnaia biokhimiia i mikrobiologiia·F XuH Li
Aug 30, 2019·Materials·Fatemeh RezaeiNathalie De Geyter
Apr 7, 2018·Applied Microbiology and Biotechnology·Zhengming ZhuGuocheng Du
Sep 8, 2018·Nature Communications·Bo ZhengYi-Xin Huo
Feb 9, 2019·Microbial Cell Factories·Huan YuYi-Xin Huo
Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tingting LiWenhui Wu

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