Novel myosin heavy chain immunohistochemical double staining developed for the routine diagnostic separation of I, IIA and IIX fibers

Acta Neuropathologica
Olayinka RaheemBjarne Udd

Abstract

The different histochemical ATPase properties of myosins separating the muscle fiber types have been utilized in diagnostic muscle biopsy routine for more than four decades. The ATPase staining method is rather laborious and has several disadvantages, such as weakening of staining over time and non-specific staining of capillaries, making the distinction of extremely atrophic muscle fibers difficult. We have developed a reliable and advanced immunohistochemical myosin double staining method for the identification of fiber types, including highly atrophic fibers in routine diagnostics. With this double staining method, we are able to distinguish among type I (ATPase type 1), IIA (ATPase type 2A), IIX (ATPase type 2B) and remodeled ATPase type 2C fibers expressing both fast and slow myosins using a one slide technique. Immunohistochemical double staining of myosin heavy chain isoforms can be used as an alternative for the conventional ATPase staining method in routine histopathology. The method provides even more detailed information of fast fiber subtypes and highly atrophic fibers on one single slide.

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Citations

Aug 2, 2011·Journal of Neurology·G MeolaR Cardani
Sep 22, 2012·Lancet Neurology·Bjarne Udd, Ralf Krahe
Apr 27, 2010·Brain : a Journal of Neurology·Homa TajsharghiBjarne Udd
Apr 9, 2014·Acta neurologica Belgica·P Y K Van den BerghE Schmedding
Feb 6, 2016·Acta Neuropathologica Communications·Satu SandellBjarne Udd
Aug 14, 2015·The American Journal of Pathology·Sanna HuovinenBjarne Udd
May 23, 2013·Journal of Plastic Surgery and Hand Surgery·Kun HwangDae Joong Kim
Dec 24, 2013·Neuromuscular Disorders : NMD·Sini PenttiläBjarne Udd
Jun 11, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Takanori ShigaHiroyuki Nakayama
Jan 5, 2018·Brain and Behavior·Laura KytövuoriMika H Martikainen

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