Novel nonsense mutation in the katA gene of a catalase-negative Staphylococcus aureus strain

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Jaime LagosJorge Fernández

Abstract

We report the first description of a rare catalase-negative strain of Staphylococcus aureus in Chile. This new variant was isolated from blood and synovial tissue samples of a pediatric patient. Sequencing analysis revealed that this catalase-negative strain is related to ST10 strain, which has earlier been described in relation to S. aureus carriers. Interestingly, sequence analysis of the catalase gene katA revealed presence of a novel nonsense mutation that causes premature translational truncation of the C-terminus of the enzyme leading to a loss of 222 amino acids. Our study suggests that loss of catalase activity in this rare catalase-negative Chilean strain is due to this novel nonsense mutation in the katA gene, which truncates the enzyme to just 283 amino acids.

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Datasets Mentioned

BETA
AJ000472
KM036425

Methods Mentioned

BETA
PCR

Software Mentioned

Chromas Lite
Bioedit

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