PMID: 8955304Dec 1, 1996Paper

Novel nuclear magnetic resonance spectroscopy methods demonstrate preferential carbon source utilization by Acinetobacter calcoaceticus

Journal of Bacteriology
G L GainesE L Neidle

Abstract

Novel nuclear magnetic resonance spectroscopy techniques, designated metabolic observation, were used to study aromatic compound degradation by the soil bacterium Acinetobacter calcoaceticus. Bacteria which had been rendered spectroscopically invisible by growth with deuterated (2H) medium were used to inoculate cultures in which natural-abundance 1H hydrogen isotopes were provided solely by aromatic carbon sources in an otherwise 2H medium. Samples taken during the incubation of these cultures were analyzed by proton nuclear magnetic resonance spectroscopy, and proton signals were correlated with the corresponding aromatic compounds or their metabolic descendants. This approach allowed the identification and quantitation of metabolites which accumulated during growth. This in vivo metabolic monitoring facilitated studies of catabolism in the presence of multiple carbon sources, a topic about which relatively little is known. A. calcoaceticus initiates aromatic compound dissimilation by forming catechol or protocatechuate from a variety of substrates. Degradation proceeds via the beta-ketoadipate pathway, comprising two discrete branches that convert catechol or protocatechuate to tricarboxylic acid cycle intermediates. As show...Continue Reading

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Citations

Sep 28, 2004·Proteomics·Seung Il KimChulhyun Lee
Apr 9, 2013·Applied and Environmental Microbiology·Christopher A Gulvik, Alison Buchan
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Becky M BundyEllen L Neidle
Mar 10, 2004·Applied and Environmental Microbiology·Alison BuchanMary Ann Moran
Nov 28, 2001·Applied and Environmental Microbiology·A BuchanM A Moran
Mar 7, 2006·Applied and Environmental Microbiology·Obidimma C EzezikaEllen L Neidle
May 8, 2007·Applied Microbiology and Biotechnology·M VeselýM Pátek
Dec 13, 2006·Applied and Environmental Microbiology·Ki Young ChoiEungbin Kim
Nov 7, 2006·Applied and Environmental Microbiology·Simone Yasmin SiehlerUlrike Gerischer
Aug 11, 2020·Metabolic Engineering Communications·Julie E ChavesJoshua K Michener

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