Novel nuclear protein ALC-INTERACTING PROTEIN1 is expressed in vascular and mesocarp cells in Arabidopsis

Journal of Integrative Plant Biology
Fang WangWei-Cai Yang

Abstract

Pod shattering is an agronomical trait that is a result of the coordinated action of cell differentiation and separation. In Arabidopsis, pod shattering is controlled by a complex genetic network in which ALCATRAZ (ALC), a member of the basic helix-loop-helix family, is critical for cell separation during fruit dehiscence. Herein, we report the identification of ALC-INTERACTING PROTEIN1 (ACI1) via the yeast two-hybrid screen. ACI1 encodes a nuclear protein with a lysine-rich domain and a C-terminal serine-rich domain. ACI1 is mainly expressed in the vascular system throughout the plant and mesocarp of the valve in siliques. Our data showed that ACI1 interacts strongly with the N-terminal portion of ALC in yeast cells and in plant cells in the nucleus as demonstrated by bimolecular fluorescence complementation assay. Both ACI1 and ALC share an overlapping expression pattern, suggesting that they likely function together in planta. However, no detectable phenotype was found in plants with reduced ACI1 expression by RNA interference technology, suggesting that ACI1 may be redundant. Taken together, these data indicate that ALC may interact with ACI1 and its homologs to control cell separation during fruit dehiscence in Arabidopsis.

References

Aug 1, 1990·The Plant Cell·D R SmythE M Meyerowitz
Apr 28, 2000·Nature·S J LiljegrenM F Yanofsky
Oct 14, 2000·Annual Review of Cell and Developmental Biology·C GrandoriR N Eisenman
Jun 13, 2001·Plant Physiology·S E Patterson
Dec 19, 2001·Current Biology : CB·S Rajani, V Sundaresan
Sep 27, 2002·Journal of Experimental Botany·Cristina Ferrándiz
Apr 4, 2003·Genes & Development·Viswanathan ChinnusamyJian-Kang Zhu
Aug 5, 2003·The Plant Cell·Gabriela Toledo-OrtizPeter H Quail
Mar 24, 2004·Cell·Sarah J LiljegrenMartin F Yanofsky
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Michael WalterJörg Kudla
Dec 22, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·José R Dinneny, Martin F Yanofsky
Aug 2, 2005·The Plant Cell·Naomi NakayamaVivian F Irish
Dec 21, 2006·Plant Biotechnology Journal·Lars ØstergaardMartin F Yanofsky

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Citations

Aug 2, 2013·Plant Cell Reports·Sylwia Struk, Pankaj Dhonukshe
Aug 2, 2011·The Plant Journal : for Cell and Molecular Biology·Michael GroszmannDavid R Smyth

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