Novel "omics" approach for study of low-abundance, low-molecular-weight components of a complex biological tissue: regional differences between chorionic and basal plates of the human placenta

Analytical and Bioanalytical Chemistry
Komal KediaSteven W Graves

Abstract

Tissue proteomics has relied heavily on two-dimensional gel electrophoresis, for protein separation and quantification, then single protein isolation, trypsin digestion, and mass spectrometric protein identification. Such methods are predominantly used for study of high-abundance, full-length proteins. Tissue peptidomics has recently been developed but is still used to study the most highly abundant species, often resulting in observation and identification of dozens of peptides only. Tissue lipidomics is likewise new, and reported studies are limited. We have developed an "omics" approach that enables over 7,000 low-molecular-weight, low-abundance species to be surveyed and have applied this to human placental tissue. Because the placenta is believed to be involved in complications of pregnancy, its proteomic evaluation is of substantial interest. In previous research on the placental proteome, abundant, high-molecular-weight proteins have been studied. Application of large-scale, global proteomics or peptidomics to the placenta have been limited, and would be challenging owing to the anatomic complexity and broad concentration range of proteins in this tissue. In our approach, involving protein depletion, capillary liquid chr...Continue Reading

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Citations

Mar 13, 2016·American Journal of Obstetrics and Gynecology·Komal KediaSteven W Graves
Dec 2, 2017·Journal of Cellular and Molecular Medicine·Antti M HaapalainenMika Rämet
May 1, 2017·Journal of Dairy Science·M A CrookendenM D Mitchell
Sep 30, 2020·Placenta·Leen J LuytenFlorence Debacq-Chainiaux
Jan 20, 2020·Analytical Biochemistry·M A El-SamahyFeng Wang
Jul 3, 2021·Frontiers in Cell and Developmental Biology·Adam JaremekStephen J Renaud
Jul 17, 2021·Journal of Proteome Research·Rachel E ForemanRichard G Kay
Nov 18, 2021·The Analyst·Takayuki KawaiKoji Otsuka

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