Novel optical PVC probes for on-site detection/determination of fluoroquinolones in a solid/liquid interface: application to the determination of Norfloxacin in aquaculture water

Biosensors & Bioelectronics
Tâmara I B SilvaM Goreti F Sales

Abstract

A novel optical disposable probe for screening fluoroquinolones in fish farming waters is presented, having Norfloxacin (NFX) as target compound. The colorimetric reaction takes place in the solid/liquid interface consisting of a plasticized PVC layer carrying the colorimetric reagent and the sample solution. NFX solutions dropped on top of this solid-sensory surface provided a colour change from light yellow to dark orange. Several metals were tested as colorimetric reagents and Fe(III) was selected. The main parameters affecting the obtained colour were assessed and optimised in both liquid and solid phases. The corresponding studies were conducted by visible spectrophotometry and digital image acquisition. The three coordinates of the HSL model system of the collected image (Hue, Saturation and Lightness) were obtained by simple image management (enabled in any computer). The analytical response of the optimised solid-state optical probe against concentration was tested for several mathematical transformations of the colour coordinates. Linear behaviour was observed for logarithm NFX concentration against Hue+Lightness. Under this condition, the sensor exhibited a limit of detection below 50 μM (corresponding to about 16 mg/...Continue Reading

References

Aug 11, 2005·The Science of the Total Environment·K G Karthikeyan, Michael T Meyer
Nov 4, 2006·Analytical and Bioanalytical Chemistry·Yolanda Picó, Vicente Andreu
Jun 2, 2007·Journal of Zhejiang University. Science. B·Yan-zhen ZhangYan Zhu
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Apr 15, 2007·Talanta·Paula C A JerónimoM Conceição B S M Montenegro

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Citations

Dec 3, 2014·Biosensors & Bioelectronics·Helena I A S Gomes, M Goreti F Sales
May 23, 2015·Journal of Aquatic Animal Health·Kun HuXian-Le Yang
Jul 11, 2019·Analytical and Bioanalytical Chemistry·Qiuju ZhouKejun Tan

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