Novel organotypic culture model of cholangiocarcinoma progression.

Hepatology Research : the Official Journal of the Japan Society of Hepatology
Deanna J W CampbellAlphonse E Sirica

Abstract

  Recent studies have suggested that increased α-smooth muscle-actin positive myofibroblastic cells (α-SMA positive CAF) in the desmoplastic stroma may relate to a more aggressive cancer and worse survival outcomes for intrahepatic cholangiocarcinoma (ICC) patients. To facilitate investigating cellular and molecular interactions between α-SMA positive CAF and cholangiocarcinoma cells related to ICC progression, we developed a novel 3-D organotypic culture model of cholangiocarcinoma that more accurately mimics the stromal microenvironment, gene expression profile and select pathophysiological characteristics of desmoplastic ICC in vivo.   This unique model was established by co-culturing within a type I collagen gel matrix, a strain of cholangiocarcinoma cells (derived from an ICC formed in syngeneic rat liver following bile duct inoculation of spontaneously-transformed rat cholangiocytes) with varying numbers of clonal α-SMA positive CAF established from the same tumor type.   Cholangiocarcinoma cells and α-SMA positive CAF in monoculture each exhibited cell-specific biomarker gene expression profiles characteristic of stromal myofibroblastic cell versus malignant cholangiocyte cell types. In comparison, the gene expression pr...Continue Reading

References

Feb 13, 2003·Nucleic Acids Research·Rafael A IrizarryTerence P Speed
Feb 3, 2006·International Journal of Cancer. Journal International Du Cancer·Kunio Matsumoto, Toshikazu Nakamura
Dec 18, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alphonse E SiricaJennifer L Dewitt
Oct 16, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alphonse E SiricaNicholas F Larusso
Dec 17, 2008·Fibrogenesis & Tissue Repair·Athina-Myrto Chioni, Richard Grose
Jun 24, 2009·Annals of Surgical Oncology·Hirohisa OkabeHideo Baba
Jul 30, 2009·Oncology Reports·Sin Young ParkWoo Sung Moon
Sep 17, 2009·The International Journal of Developmental Biology·Olivier De WeverMarc Bracke
Dec 5, 2009·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Alphonse E SiricaJennifer L Dewitt
Jan 20, 2010·Journal of Biotechnology·Fieke E M FroelingHemant M Kocher
Nov 3, 2010·Annals of Surgical Oncology·Hirohisa OkabeHideo Baba
Feb 8, 2011·Current Opinion in Gastroenterology·Alphonse E SiricaCatherine I Dumur
Dec 7, 2011·Nature Reviews. Gastroenterology & Hepatology·Alphonse E Sirica

❮ Previous
Next ❯

Citations

Dec 3, 2014·Experimental and Molecular Pathology·Alphonse E SiricaChao Li
Oct 22, 2013·Gastroenterology·Sumera Rizvi, Gregory J Gores
Apr 5, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Runping LiuHuiping Zhou
Oct 15, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alphonse E Sirica, Gregory J Gores
Dec 18, 2013·The American Journal of Pathology·Kan HandaAlejandro Soto-Gutierrez
Jul 31, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ling LiFlorin M Selaru
Dec 14, 2016·Annual Review of Pathology·Silvia AffoRobert F Schwabe
Oct 18, 2016·Journal of Tissue Engineering·Tuula M ErikssonVehid M Salih
Mar 10, 2019·Liver International : Official Journal of the International Association for the Study of the Liver·Silvestre VicentVincenzo Cardinale
Apr 12, 2017·World Journal of Hepatology·Simone BrivioLuca Fabris
Jan 15, 2020·Current Opinion in Gastroenterology·Javier VaqueroLaura Fouassier
Feb 26, 2013·Current Opinion in Gastroenterology·Kwang Suk KoHeping Yang
Jan 11, 2020·Frontiers in Medicine·Sukanya RoySanjukta Chakraborty
Jul 2, 2020·Nature Reviews. Gastroenterology & Hepatology·Jesus M BanalesGregory J Gores
Apr 10, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Malinee ThaneePuangrat Yongvanit
Aug 23, 2020·Cancers·Annamaria MassaMassimo Aglietta
Dec 12, 2012·Cancer Research·Joachim C MertensGregory J Gores
Mar 23, 2013·Disease Models & Mechanisms·Abigail ZabronShahid A Khan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Hajime IsomotoGregory J Gores
Proceedings of the National Academy of Sciences of the United States of America
Hai SongYingzi Yang
International Journal of Cancer. Journal International Du Cancer
Tania M WelzelKatherine A McGlynn
© 2021 Meta ULC. All rights reserved