Novel pathways of F-actin polymerization in the human neutrophil.

Blood
David Chodniewicz, Doncho V Zhelev

Abstract

Recently we demonstrated the existence of a phosphatidylinositol 3-kinase (PI3K)-independent F-actin polymerization during neutrophil pseudopod extension. Here we examine the use of the PI3K-dependent and PI3K-independent pathways of activation by the N-formyl peptide receptor and the chemokine receptors, and the priming of the 2 pathways by granulocyte-macrophage colony-stimulating factor (GM-CSF) and insulin. The inhibition of PI3K activity with wortmannin showed that rate of pseudopod extension stimulated with N-formyl-Met-Leu-Phe (fMLP was mostly dependent on PI3K, while the rate of interleukin-8 (IL-8)-stimulated pseudopod extension was less dependent on PI3K. The incubation of cells with either GM-CSF or insulin increased the rate of pseudopod extension by 50% when the cells were stimulated with IL-8 but not with fMLP. The stimulation with IL-8 phosphorylated the PI3K regulatory subunit. This phosphorylation was enhanced by GM-CSF, which increased PI3K activity and total phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) production. The effect of GM-CSF was blocked with wortmannin. In contrast, insulin did not increase p85 phosphorylation and did not enhance PI3K activity or PtdIns(3,4,5)P3 production. The effect ...Continue Reading

References

Mar 1, 1995·Immunopharmacology·L A BoberS K Narula
Sep 6, 1996·The Journal of Biological Chemistry·H Arai, I F Charo
May 19, 1998·Life Sciences·O HazekiT Katada
Oct 6, 1998·Annual Review of Biochemistry·D A FrumanL C Cantley
Aug 5, 1999·Cellular Signalling·F H Nystrom, M J Quon
Dec 22, 1999·The Journal of Biological Chemistry·B HaribabuR Snyderman
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·T D PollardR D Mullins
May 5, 2001·The Journal of Biological Chemistry·B D CuevasG B Mills
Jun 8, 2001·Annual Review of Biochemistry·B VanhaesebroeckM D Waterfield
Jun 14, 2001·Biochemistry. Biokhimii︠a︡·V L Katanaev
Sep 5, 2001·The Journal of Biological Chemistry·C C CunninghamP A Janmey
Mar 14, 2002·Current Opinion in Cell Biology·Orion D Weiner
Jun 8, 2002·Annals of Biomedical Engineering·Doncho V Zhelev, Abdullatif Alteraifi

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Citations

May 17, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Wenying ZouFei Zou
Feb 4, 2010·Cardiovascular Research·Connie H Y WongPaul Kubes
Jul 13, 2006·Annual Review of Biomedical Engineering·Geert W Schmid-Schönbein
Jun 1, 2012·Expert Opinion on Drug Discovery·Cailong FangSun Ying
Jul 21, 2006·Annals of the New York Academy of Sciences·Fabrizio MontecuccoLuciano Ottonello
Mar 27, 2007·Annals of the New York Academy of Sciences·Fabrizio MontecuccoLuciano Ottonello
Oct 6, 2005·Biochemical and Biophysical Research Communications·Manya Dhar-MascarenoDavid W Golde
Jul 13, 2004·Biochimica Et Biophysica Acta·David Chodniewicz, Richard L Klemke
Jul 9, 2004·Biophysical Journal·Doncho V ZhelevDavid Chodniewicz
Mar 2, 2005·European Journal of Immunology·Matthew J ThomasJohn Westwick
Oct 2, 2012·Oncology Reports·Magdalena IzdebskaAlina Grzanka
Dec 17, 2004·Pharmacological Reviews·Peter J Barnes
Jun 16, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Giovanni BernardiniAngela Santoni
Jul 25, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Simon McArthurEgle Solito
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lorena Riol-BlancoJosé Luis Rodríguez-Fernández
Aug 18, 2020·Journal of Ethnopharmacology·Kuei-Hung LaiTsong-Long Hwang
Jan 12, 2008·Journal of Cell Science·Bryan HeitPaul Kubes
Mar 18, 2020·Biochemistry·Natasha A MoussourasMichael B Dwinell

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