PMID: 9171093Jun 15, 1997Paper

Novel pattern of DNA methylation in Neurospora crassa transgenic for the foreign gene hph

Nucleic Acids Research
A C CodónV E Russo

Abstract

It has previously been reported that multiple copies of the hph gene integrated into the genome of Neurospora crassa are methylated at Hpa II sites (CCGG) during the vegetative life cycle of the fungus, while hph genes integrated as single copies are not methylated. Furthermore, methylation is correlated with silencing of the gene. We report here the methylation state of cytosine residues of the major part of the promoter region of the hph gene integrated into the genome of the multiple copy strain HTA5.7 during the vegetative stage of the life cycle. Cytosine methylation is sequence dependent, but the sequence specificity is complex and is different from the sequence specificity known for mammals and plants (CpG and CpNpG). The pattern of DNA methylation reported here is very different from that measured after meiosis in Neurospora or in Ascobulus . After the sexual cycle in those two fungi all the cytosines of multiple stretches of DNA are heavily methylated. This indicates that the still unknown methyltransferase in Neurospora has a different specificity in the sexual and the vegetative stages of the life cycle or that there are different methyltransferases. The pattern of methylation reported here is also different from the...Continue Reading

References

Mar 20, 1995·Analytical Biochemistry·A M RaizisJ P Jost
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·I IngelbrechtA Depicker
Sep 1, 1994·Molecular and Cellular Biology·E S Tasheva, D J Roufa
Aug 11, 1994·Nucleic Acids Research·S J ClarkM Frommer
Jun 1, 1996·Molecular and Cellular Biology·C GoyonJ L Rossignol
Apr 11, 1997·The Journal of Biological Chemistry·T ReinH Zorbas

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