Novel phosphorylcholine-containing protein of Pseudomonas aeruginosa chronic infection isolates interacts with airway epithelial cells

The Journal of Infectious Diseases
Mariette BarbierSebastián Albertí

Abstract

Pseudomonas aeruginosa undergoes phase variation in the expression of the phosphorylcholine (ChoP) epitope, a structure crucial for the virulence of several respiratory pathogens. In this study, ChoP expression analysis comparing organisms from acute and chronic infections revealed that expression of ChoP at 37 degrees C was higher among strains from chronic infections. Coimmunoprecipitation experiments and mass spectrometry analysis demonstrated that ChoP was on the protein elongation factor Tu. The presence of ChoP at the surface was confirmed by immunofluorescence and flow cytometry analysis of intact bacteria. Pretreatment of bronchial epithelial cells or mice with a platelet-activating factor receptor (PAFR) antagonist reduced adhesion and invasion of the ChoP-positive P. aeruginosa isolates. Results of this study suggest that ChoP expression may represent a novel phenotype expressed by the chronic infection isolates that could mediate P. aeruginosa colonization of the epithelial airway by means of the interaction with the PAFR.

References

May 6, 1976·Nature·G R Jacobson, J P Rosenbusch
Aug 1, 1996·The European Respiratory Journal·S A EvansM A Woodhead
Apr 14, 1999·Molecular Microbiology·J R ZhangE I Tuomanen
Apr 5, 2002·Clinical Microbiology Reviews·Jeffrey B LyczakGerald B Pier
Jun 20, 2003·Lancet·Jean-Louis Vincent
Feb 10, 2004·The Journal of Infectious Diseases·Anita W RijneveldTom van der Poll
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·Maria D MaciáAntonio Oliver
Feb 8, 2005·American Journal of Respiratory and Critical Care Medicine·Ruxana T SadikotAlice S Prince
May 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Dao Nguyen, Pradeep K Singh
Feb 10, 2007·Vaccine·Sarah V HardingRichard W Titball

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Citations

Feb 18, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Justin A ThorntonElaine I Tuomanen
Sep 12, 2012·The Journal of Infectious Diseases·Mariette BarbierSebastián Albertí
Dec 12, 2012·Infection and Immunity·Sarah E Clark, Jeffrey N Weiser
Jun 5, 2014·Journal of Infection and Public Health·Ghada F AhmedAyman M Noreddin
Jan 1, 2013·Biochemical and Biophysical Research Communications·Freda E-C JenMichael P Jennings
Apr 1, 2011·Environmental Microbiology·Sophie de Bentzmann, Patrick Plésiat
Dec 18, 2015·The Journal of Biological Chemistry·Joshua P OwingsJoanna B Goldberg
Feb 14, 2018·The European Respiratory Journal·Lisa MiyashitaJonathan Grigg
Jul 14, 2016·The Biochemical Journal·Pål Ø FalnesJędrzej Małecki
Oct 31, 2019·Respirology : Official Journal of the Asian Pacific Society of Respirology·Shakti D ShuklaPhilip M Hansbro
Nov 23, 2019·The Journal of Biological Chemistry·Emily G KuiperGraeme L Conn
Dec 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ronan LordanConstantinos A Demopoulos
May 28, 2021·European Respiratory Review : an Official Journal of the European Respiratory Society·E Haydn WaltersChris Ward

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