Novel plasma peptide markers involved in the pathology of CKD identified using mass spectrometric approach

Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte
Prathibha R GajjalaVera Jankowski

Abstract

Chronic kidney disease (CKD) may progress to end-stage renal disease (ESRD) at different pace. Early markers of disease progression could facilitate and improve patient management. However, conventional blood and urine chemistry have proven unable to predict the progression of disease at early stages. Therefore, we performed untargeted plasma peptidome analysis to select the peptides involved in progression, which are suitable for long prospective studies in future. The study consists of non-CKD (n = 66) and CKD (n = 106) patients with different stages. We performed plasma peptidomics on these subjects using chromatography and mass spectrometric approaches. Initially, we performed LC-ESI-MS and applied least absolute shrinkage and selection operator logistic regressions to select the peptides that are differentially expressed and we generated a peptidomic score for each subject. Later, we identified and sequenced the peptides with MALDI-MS/MS and also performed univariate and multivariate analyses with the clinical variables and peptidomic score to reveal their association with progression of renal disease. A logistic regression model selected 14 substances showing different concentrations according to renal function, of which ...Continue Reading

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Citations

Jul 25, 2021·International Journal of Molecular Sciences·Joanna Tracz, Magdalena Luczak
Jul 17, 2021·Journal of Proteome Research·Rachel E ForemanRichard G Kay

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Methods Mentioned

BETA
biopsy

Software Mentioned

Proteome Discoverer
HyStar
Compass
LASSO
RapideNovo
R package MICE
Mascot
Statistical Analysis System ” ( SAS )

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