Novel plasmid-free Gluconobacter oxydans strains for production of the natural sweetener 5-ketofructose

Microbial Cell Factories
Svenja BattlingMichael Bott

Abstract

5-Ketofructose (5-KF) has recently been identified as a promising non-nutritive natural sweetener. Gluconobacter oxydans strains have been developed that allow efficient production of 5-KF from fructose by plasmid-based expression of the fructose dehydrogenase genes fdhSCL of Gluconobacter japonicus. As plasmid-free strains are preferred for industrial production of food additives, we aimed at the construction of efficient 5-KF production strains with the fdhSCL genes chromosomally integrated. For plasmid-free 5-KF production, we selected four sites in the genome of G. oxydans IK003.1 and inserted the fdhSCL genes under control of the strong P264 promoter into each of these sites. All four recombinant strains expressed fdhSCL and oxidized fructose to 5-KF, but site-specific differences were observed suggesting that the genomic vicinity influenced gene expression. For further improvement, a second copy of the fdhSCL genes under control of P264 was inserted into the second-best insertion site to obtain strain IK003.1::fdhSCL2. The 5-KF production rate and the 5-KF yield obtained with this double-integration strain were considerably higher than for the single integration strains and approached the values of IK003.1 with plasmid-ba...Continue Reading

References

Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 13, 2001·Journal of Applied Microbiology·J C BarbeA Lonvaud-Funel
Feb 15, 2001·Biochemical Engineering Journal·T Anderlei, J Büchs
Nov 19, 2002·Applied Microbiology and Biotechnology·U DeppenmeierC Prust
Jan 25, 2005·Nature Biotechnology·Christina PrustUwe Deppenmeier
May 9, 2007·Nucleic Acids Research·Andreas UntergasserJack A M Leunissen
May 15, 2007·Applied Microbiology and Biotechnology·Cornelia GätgensUte Herrmann
Aug 19, 2007·Journal of Molecular Microbiology and Biotechnology·Paul SchweigerUwe Deppenmeier
Sep 13, 2007·Critical Reviews in Biotechnology·Cassandra De MuynckErick J Vandamme
Aug 30, 2008·Journal of Agricultural and Food Chemistry·Mélanie BlasiHervé Deleuze
Oct 30, 2008·Journal of Molecular Microbiology and Biotechnology·Tina HölscherHermann Sahm
Oct 30, 2008·Journal of Molecular Microbiology and Biotechnology·Uwe Deppenmeier, Armin Ehrenreich
Dec 6, 2008·The American Journal of Clinical Nutrition·Richard D Mattes, Barry M Popkin
Feb 9, 2010·Physiology & Behavior·Frank B Hu, Vasanti S Malik
Oct 19, 2010·Current Opinion in Microbiology·Douglas F BrowningStephen Jw Busby
Oct 26, 2010·Journal of Biotechnology·Verena KallnikPaul Schweiger
Feb 2, 2011·RNA Biology·Magali NavilleDaniel Gautheret
May 24, 2011·Methods in Enzymology·Daniel G Gibson
May 24, 2012·Journal of Agricultural and Food Chemistry·Caroline HellfritschThomas Hofmann
Jul 14, 2012·Applied Microbiology and Biotechnology·Maria MeyerUwe Deppenmeier
Sep 4, 2012·Applied Microbiology and Biotechnology·Björn PetersArmin Ehrenreich
Jan 29, 2013·Applied Microbiology and Biotechnology·Janine RichhardtMichael Bott
Feb 5, 2014·JAMA Internal Medicine·Quanhe YangFrank B Hu
Dec 9, 2014·Biotechnology Advances·Natsaran SaichanaJitka Frebortova
Feb 2, 2015·Current Opinion in Microbiology·Robert LandickDavid C Grainger
Mar 31, 2016·Nature Communications·Avantika LalSankar Adhya
May 10, 2017·Applied Microbiology and Biotechnology·Ines KieflerMichael Bott
May 17, 2017·Biophysics Reviews·Charles J Dorman, Matthew J Dorman
Jul 10, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Márcio CarochoIsabel C F R Ferreira
Dec 10, 2017·Scientific Reports·Peter GroßmannChristoph Kaleta
Dec 28, 2017·Applied Microbiology and Biotechnology·Anna SiemenUwe Deppenmeier
Jan 7, 2018·BMC Genomics·Angela KranzTino Polen
Dec 6, 2018·Nucleic Acids Research·Dmitry SutorminKonstantin Severinov

❮ Previous
Next ❯

Methods Mentioned

BETA
RNAseq
PCR
DNA folding
electrophoresis

Software Mentioned

ARNold
RAMOS
Respiration Activity MOnitoring System ( RAMOS
Primer3plus
Respiration Activity MOnitoring System ( RAMOS )

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.