Novel poly(amidoamine)s with pendant primary amines as highly efficient gene delivery vectors

Macromolecular Bioscience
Min LiuShi-Wen Huang

Abstract

Three poly(amidoamine)s with pendant primary amines were synthesized by Michael polyaddition of a diamine to N,N-methylenebis(acrylamide). The poly(amidoamine)s with primary and tertiary amines possess a high buffer capacity between pH = 5 and 7, which facilitates the escape of polymer/DNA complexes from endosomes according to the 'proton sponge hypothesis'. In vitro cytotoxicity of poly(amidoamine)s depends on the side-chain structure of the polymer. Polymers 1a-1c can condense plasmid DNA by electrostatic interactions to form nanosized polyelectrolyte complexes with a positive surface charge. In vitro transfection results indicate that the transfection efficiency of polymer 1b under optimized conditions is comparable to that of commercial branched PEI 25,000.

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Jan 13, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hajar SeyednejadWim E Hennink
Jan 31, 2017·ACS Applied Materials & Interfaces·Gang ChenDavid Oupický
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Oct 24, 2013·Macromolecular Rapid Communications·Katrin RoemhildThomas Heinze
Apr 11, 2012·The Journal of Organic Chemistry·Felix WojcikLaura Hartmann

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